2024-03-29T04:40:57Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/121472016-11-23T08:38:36Zcom_10261_75com_10261_6col_10261_1210
00925njm 22002777a 4500
dc
Muñoz-Amatriaín, María
author
Svensson, J. T.
author
Castillo Alonso, Ana María
author
Cistué Sola, Luis
author
Close, T. J.
author
Vallés Brau, María Pilar
author
2009
In barley, microarray technology has allowed to study the gene expression profiling associated with the stress pretreatment phase of microspore embryogenesis, where the reprogramming of microspores takes place. Transcriptome analysis of anthers before and after four days of mannitol pretreatment, revealed changes in the expression of 2,673 genes. A thorough study of these differentially expressed genes indicated that microspores maintained their cell cycle machinery in a steady state during stress pretreatment and underwent a major reorganization of metabolic pathways accompanied by a multi-dimensional stress response. Up-regulation of transcription factors related to stress responses and changes in developmental programmes took place during the pretreatment. Preliminary studies have indicated that YABBY5, ZML2, CURLY LEAF and ICE1 transcription factors have a stress pretreatment specific induction and, therefore, could play a direct role in microspore reprogramming.
Muñoz-Amatriaín, M. [et al.], Expression Profiles in Barley Microspore Embryogenesis. In: Touraev, A., Forster, B.P., Jain, S.M. (eds.), Advances in Haploid Production in Higher Plants, Springer, 2009, pp.: 127-134. ISBN: 978-1-4020-8853-7.
978-1-4020-8853-7 (Print)
978-1-4020-8854-4 (Online)
http://hdl.handle.net/10261/12147
10.1007/978-1-4020-8854-4_9
Barley
Expression profiling
Microarray
Microspore embryogenesis
Reprogramming
Stress pretreatment
Transcription factors
Expression profiles in barley microspore embryogenesis