2024-03-28T12:06:48Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1029022021-12-28T16:19:27Zcom_10261_47com_10261_8col_10261_300
00925njm 22002777a 4500
dc
García-Valtanen, Pablo
author
Ortega-Villaizán, M. del Mar
author
Martínez-López, Alicia
author
Medina-Gali, Regla María
author
Pérez, Luis
author
MacKenzie, Simon
author
Figueras Huerta, Antonio
author
Coll, Julio
author
Estepa, Amparo
author
2014
It has not been elucidated whether or not autophagy is induced by rhabdoviral G glycoproteins (G) in vertebrate organisms for which rhabdovirus infection is lethal. Our work provides the first evidence that both mammalian (vesicular stomatitis virus, VSV) and fish (viral hemorrhagic septicemia virus, VHSV, and spring viremia carp virus, SVCV) rhabdoviral Gs induce an autophagic antiviral program in vertebrate cell lines. The transcriptomic profiles obtained from zebrafish genetically immunized with either Gsvcv or Gvhsv suggest that autophagy is induced shortly after immunization and therefore, it may be an important component of the strong antiviral immune responses elicited by these viral proteins. Pepscan mapping of autophagy-inducing linear determinants of Gvhsv and Gvsv showed that peptides located in their fusion domains induce autophagy. Altogether these results suggest that strategies aimed at modulating autophagy could be used for the prevention and treatment of rhabdoviral infections such as rabies, which causes thousands of human deaths every year.
Autophagy 10(9): 1666-1680 (2014)
1554-8627
http://hdl.handle.net/10261/102902
10.4161/auto.29557
1554-8635
25046110
Antiviral
Autophagy
Immune response
LC3
Microarrays
Pepscan
Rhabdovirus
SVCV
VHSV
Viral glycoprotein
VSV
Zebrafish
Autophagy-inducing peptides from mammalian VSV and fish VHSV rhabdoviral G glycoproteins (G) as models for the development of new therapeutic molecules