2024-03-29T14:06:54Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/992142018-08-30T07:09:07Zcom_10261_63com_10261_6col_10261_442
2014-07-01T09:40:22Z
urn:hdl:10261/99214
Use of stomach tubing as an alternative to rumen cannulation to study the bacterial community in sheep and goats
Ramos Morales, Eva
Arco Pérez, Ana
Martín García, A. Ignacio
Yáñez Ruiz, David R.
Frutos, Pilar
Hervás, Gonzalo
1 página.-- Gut microbiology: from sequence to function (Aberdeen, Reino Unido, 16-19 junio, 2014).
Rumeh cannulation is considered the reference method for collection of representative samples of rumen
digesta and is widely used in ruminant nutrition research. However, it is not feasible in lactating ewes or goats,
because of adverse effects on animal performance, which obliges to depend on less invasive techniques, such
as stomach tubing. The aim of this work was to study whether collecting samples through cannula or stomach
tube detect the differences in the rumen bacterial community between species (sheep and goats) and diets
(forage and forage plus concentrate, 1:1). Four sheep and four goats, fitted with ruminal cannula, were fed
either forage or forage plus concentrate, in two 15-d periods. At the end of each period, samples of rumen
digesta were taken after feeding, first by stomach tube and immediately afterwards via rumen cannula. PCRDGGE
analysis of the bacterial community was carried out.
The similarity dendrograrn showed that, within period, animal species was the factor driving the grouping
pattem, which was more evident in samples collected through cannula. Furthermore, diversity indexes were
higher for goats than for sheep only when sampling via cannula. None of the techniques detected significant
variations in the bacterial diversity when comparing the diets. Our results show that stomach tubing allows the
collection of a highly diverse bacterial community that is able to detect some of the effects observed when
sampling through cannula. However, further studies in other microbial groups, using high-throughput
sequencing tools, are needed to explore difierences in the abundances of some microbial taxa.
2014-07-01T09:40:22Z
2014-07-01T09:40:22Z
2014-06
póster de congreso
Gut microbiology: from sequence to function 154 (P204) (2014)
http://hdl.handle.net/10261/99214
eng
Sí
openAccess
Rowett Institute of Nutrition and Health