2024-03-29T12:32:51Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/964322016-11-14T12:03:49Zcom_10261_75com_10261_6col_10261_328
2014-05-09T10:04:49Z
urn:hdl:10261/96432
Further characterization of the photoreaction center from Ectothiorhodospira sp. detection of the H subunit by monoclonal antibodies
Lefebvre, Suzie
Picorel Castaño, Rafael
Gingras, Gabriel
Photoreaction center
Monoclonal antibodies
Ectothiorhodospira sp.
The photoreaction center isolated from Ectothiorhodospira sp. is known to contain polypeptides L, M and C (a four heme c cytochrome) in a 1:1:1 molar ratio. As a test for the presence of a H subunit, Western blots of the chromatophore and photoreaction center polypeptides were reacted with a monoclonal antibody directed against the H subunit of Rhodospirillum rubrum. The chromatophores but not the isolated photoreaction center cross-reacted with this antibody. It is concluded that the H polypeptide is lost during the purification of the photoreaction center which, in its native state, therefore contains four polypeptides including a genuine H subunit.
2014-05-09T10:04:49Z
2014-05-09T10:04:49Z
1990
2014-05-09T10:04:49Z
artículo
FEMS Microbiology Letters 65 (3): 247-252 (1990)
http://hdl.handle.net/10261/96432
10.1016/0378-1097(89)90225-5
eng
http://dx.doi.org/10.1016/0378-1097(89)90225-5
closedAccess
Blackwell Publishing