2024-03-30T05:17:28Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/795192022-11-22T10:20:27Zcom_10261_22com_10261_1col_10261_275
2013-07-12T08:35:19Z
urn:hdl:10261/79519
Characterization of non-dominant lethal mutations in the yeast plasma membrane H+-ATPase gene
Martín-Castillo, Begoña
Portillo, Francisco
Site-directed mutants of yeast ATPase were previously studied after introduction of mutant alleles into a yeast strain where these alleles were constitutively expressed while the expression of the wild-type chromosomal ATPase gene was turned off. As a functional H+ pump is essential, strong selective pressure leads to the accumulation of revertants during growth of cells harboring variants with low activity. Thus, constitutive expression of the mutant gene can select phenotypes which reflect events such as gene conversion or reversion. We have therefore re-evaluated the phenotypes of non-dominant lethal alleles in an alternative set of conditional expression systems. We show that eight of 11 previously described site-directed mutations behave as recessive lethal alleles. Copyright (C) 1999 Elsevier Science B.V.
2013-07-12T08:35:19Z
2013-07-12T08:35:19Z
1999
2013-07-12T08:35:20Z
artículo
BBA - Biomembranes 1417(1): 32-36 (1999)
http://hdl.handle.net/10261/79519
10.1016/S0005-2736(98)00247-8
eng
closedAccess
Elsevier