2024-03-29T14:13:26Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/748922017-04-03T11:58:53Zcom_10261_103com_10261_1col_10261_356
2013-04-23T08:53:17Z
urn:hdl:10261/74892
Functional analyses of AGAMOUS family members in Nicotiana benthamiana clarify the evolution of early and late roles of C-function genes in eudicots
Fourquin, Chloe
Ferrándiz, Cristina
Ministerio de Ciencia e Innovación (España)
Floral homeotic gene
Mads-box genes
Controlling flower development
Organ identity
Regulatory interactions
Shatterproof genes
Ectopic expression
Fruit-development
The C-function, according to the ABC model of floral organ identity, is required for stamen and carpel development and to provide floral meristem determinacy. Members of the AG lineage of the large MADS box gene family specify the C-function in a broadly conserved manner in angiosperms. In core eudicots, two sub-lineages co-exist, euAG and PLE, which have been extensively characterized in Antirrhinum majus and Arabidopsis thaliana, where strong sub-functionalization has led to highly divergent contributions of the respective paralogs to the C-function. Various scenarios have been proposed to reconstruct the evolutionary history of the euAG and PLE lineages in eudicots, but detailed functional analyses of the roles of these genes in additional representative species to validate evolutionary hypotheses are scarce. Here, we report functional characterization of euAG- and PLE-like genes in Nicotiana benthamiana through expression analyses and phenotypic characterization of the defects caused by their specific down-regulation. We show that both paralogs redundantly contribute to the C-function in this species, providing insights on the likely evolution of these gene lineages following divergence of the major groups within the eudicots (rosids and asterids). Moreover, we have demonstrated a conserved role for the PLE-like genes in controlling fruit dehiscence, which strongly supports the ancestral role of PLE-like genes in late fruit development and suggests a common evolutionary origin of late developmental processes in dry (dehiscent) and fleshy (ripening) fruits.
2013-04-23T08:53:17Z
2013-04-23T08:53:17Z
2012-09
artículo
Plant Journal 71(6): 990-1001 (2012)
0960-7412
http://hdl.handle.net/10261/74892
10.1111/j.1365-313X.2012.05046.x
http://dx.doi.org/10.13039/501100004837
eng
Postprint
http://doi.org/10.1111/j.1365-313X.2012.05046.x
openAccess
Wiley-Blackwell