2024-03-29T12:47:04Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/722692018-07-06T10:30:49Zcom_10261_88com_10261_8col_10261_341
2013-03-15T12:46:34Z
urn:hdl:10261/72269
A NGS approach to the encrusting Mediterranean sponge Crella elegans (Porifera, Demospongiae, Poecilosclerida): transcriptome sequencing, characterization and overview of the gene expression along three life cycle stages
Perez-Porro, Alicia-R.
Navarro-Gómez, D.
Uriz, María Jesús
Giribet, Gonzalo
Poecilosclerida
Transcriptome characterization
Crella elegans
De novo assembly
Life cycle stages
Mediterranean sponge
16 páginas, 9 figuras, 9 tablas.
Sponges can be dominant organisms in many marine and freshwater habitats where they play essential ecological
roles. They also represent a key group to address important questions in early metazoan evolution. Recent
approaches for improving knowledge on sponge biological and ecological functions as well as on animal evolution
have focused on the genetic toolkits involved in ecological responses to environmental changes (biotic and abiotic),
development and reproduction. These approaches are possible thanks to newly available, massive sequencing technologies–
such as the Illumina platform, which facilitate genome and transcriptome sequencing in a cost-effective
manner. Here we present the first NGS (next-generation sequencing) approach to understanding the life cycle of an
encrusting marine sponge. For this we sequenced libraries of three different life cycle stages of the Mediterranean
sponge Crella elegans and generated de novo transcriptome assemblies. Three assemblies were based on sponge
tissue of a particular life cycle stage, including non-reproductive tissue, tissue with sperm cysts and tissue with
larvae. The fourth assembly pooled the data from all three stages. By aggregating data from all the different life cycle
stages we obtained a higher total number of contigs, contigs with BLAST hit and annotated contigs than from one
stage-based assemblies. In that multi-stage assembly we obtained a larger number of the developmental regulatory
genes known for metazoans than in any other assembly. We also advance the differential expression of selected
genes in the three life cycle stages to explore the potential of RNA-seq for improving knowledge on functional
processes along the sponge life cycle.
2013-03-15T12:46:34Z
2013-03-15T12:46:34Z
2013
artículo
Molecular Ecology Resources : DOI: 10.1111/1755-0998.12085 (2013)
1755-098X
http://hdl.handle.net/10261/72269
10.1111/1755-0998.12085
eng
http://dx.doi.org/10.1111/1755-0998.12085
openAccess
Blackwell Publishing