2024-03-29T06:59:23Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/70702021-07-28T12:07:13Zcom_10261_125com_10261_2col_10261_378
2008-09-03T08:22:30Z
urn:hdl:10261/7070
Qualitative and quantitative analyses of the bifidobacterial microbiota in the colonic mucosa of patients with colorectal cancer, diverticulitis and infl ammatory bowel disease
Gueimonde Fernández, Miguel
Ouwehand, Arthur C.
Huhtinen, Heikki
Salminen, Eeva
Salminen, Seppo
Bifidobacterias
Mucosa colónica
Cáncer colorrectal
Diverticulitis
AIM: To characterize the bifidobacterial microbiota of the colonic mucosa in patients with colon cancer, inflammatory bowel disease or diverticulitis.
METHODS: A sample of the distal colonic mucosa was taken during surgery from a total of 34 patients, twenty-one with diagnosed colorectal cancer, nine with diverticulitis and four with inflammatory bowel disease, requiring surgery for their condition. Bacterial DNA was extracted from the resected mucosal samples and bifidobacterial mucosa-associated microbiota was qualitatively and quantitatively determined by means of qualitative and quantitative PCR.
RESULTS: Bifidobacteria were found in 100% of the samples from patients with diverticulitis or IBD and a 76% of those suffering colon cancer. The species B. longum and B. bifidum were the most widely found, followed by B. animalis, B. catenulatum and B. adolescentis. B. breve, B. dentium and B. angulatum were not detected in any sample. A significantly higher occurrence of B. longum was observed in patients with diverticulitis than in those with colon cancer or IBD (100%, 62% and 75%, respectively, P < 0.05). Similar results were obtained for B. animalis (56%, 0% and 25%, P < 0.05), while B. adolescentis was only found in the mucosa from patients with colon cancer (5 out of 21, 24%). At the quantitative level, patients with colon cancer or IBD showed lower counts of total Bifidobacterium (4.94 and 5.91 vs 6.96 log Cells/sample, respectively, P < 0.05) and of the species B. longum (4.05 and 4.79 vs 6.76, P < 0.05) than those with diverticulitis.
CONCLUSION: Aberrancies in mucosa associated microbiota are present in different intestinal diseases. This may indicate a role of the microbiota in the pathogenesis of these diseases
2008-09-03T08:22:30Z
2008-09-03T08:22:30Z
2007-08-07
artículo
World Journal of Gastroenterology 13(29), 3985-3989 (2007)
1007-9327
http://hdl.handle.net/10261/7070
eng
openAccess
WJG Press