2024-03-29T09:21:21Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/528712016-02-17T09:50:36Zcom_10261_31com_10261_3col_10261_284
2012-07-06T08:02:27Z
urn:hdl:10261/52871
In situ photopolymerization of biomaterials by thiol-yne click chemistry
Lomba, Miguel
Oriol, Luis
Alcalá, Rafael
Sánchez-Somolinos, Carlos
Grazú, Valeria
Serrano, José Luis
Fuente, Jesús M. de la
The thiol-yne click chemistry reaction has been used for the in situ photocrosslinking of an aliphatic hyperbranched polyester. The biocompatibility of the resulting networks has been studied and marked cytotoxicity was not found for HeLa (human cervical carcinoma) tumoral cells and COS7 fibroblasts. The photoinduced thiol-yne process allows the generation of patterned structures with different geometries in films by DLW and these materials can be used as substrates for cell adhesion. The influence of the substrate geometry on cell adhesion has been studied by culturing cells onto these substrates and a preference for the photopatterned polymeric material can be seen in some of the structures by contrast phase microscopy. Actin and vinculin fluorescent staining revealed different adhesion behavior for HeLa cells and COS7 fibroblasts and this could be assigned to the different motility of cells. The thiol-yne photoreaction has proven to be an attractive approach for the preparation of micropatterned biomaterials.
2012-07-06T08:02:27Z
2012-07-06T08:02:27Z
2011
2012-07-06T08:02:27Z
artículo
Macromolecular Bioscience 11(11): 1505-1514 (2011)
http://hdl.handle.net/10261/52871
10.1002/mabi.201100123
eng
closedAccess
Wiley-VCH