2024-03-29T05:53:52Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/236542021-05-11T01:24:33Zcom_10261_74com_10261_6col_10261_327
2010-04-28T10:26:53Z
urn:hdl:10261/23654
Detection of the defoliating pathotype of Verticillium dahliae in infected olive plants by nested PCR
Mercado-Blanco, Jesús
Rodríguez-Jurado, Dolores
Pérez-Artés, Encarnación
Jiménez-Díaz, Rafael M.
Molecular diagnosis
Olea europaea
Pathogen
Free certification programs
Verticillium wilt
13 pages, 6 figures
Spread of Verticillium wilt into newly established olive orchards in Andaluc´ıa, southern Spain, has caused concern
in the olive industry in the region. This spread may result from use of Verticillium dahliae-infected planting material,
which can extend distribution of the highly virulent, defoliating (D) pathotype of V. dahliae to newareas. In this study,
a molecular diagnostic method for the early in planta detection of D V. dahliae was developed, aimed especially
at nursery-produced olive plants. For this purpose, new primers for nested PCR were designed by sequencing a
992-bp RAPDmarker of theDpathotype. The use of the specific primers and different nested-PCR protocols allowed
the detection of V. dahliae pathotype D DNA in infected root and stem tissues of young olive plants. Detection of
the pathogen was effective from the very earliest moments following inoculation of olive plants with a V. dahliae pathotype D conidia suspension as well as in inoculated, though symptomless, plants.
2010-04-28T10:26:53Z
2010-04-28T10:26:53Z
2002-01
artículo
European Journal of Plant Pathology (2002), 108: 1–13
0929-1873
http://hdl.handle.net/10261/23654
10.1023/A:1013994827836
1573-8469
http://dx.doi.org/10.1023/A:1013994827836
closedAccess
Springer