2024-03-28T17:37:41Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/224522019-02-28T12:10:16Zcom_10261_5063com_10261_5col_10261_5066
2010-03-17T09:16:48Z
urn:hdl:10261/22452
High-level production of recombinant His-tagged rhamnulose 1-phosphate aldolase in Escherichia coli
Vidal, Luis
Durany, Olga
Suau, T.
Ferrer, Pau
Benaiges, M. D.
Caminal, Glòria
Recombinant aldolase
His-tagged
Purification
Bioreactor
E coli
9 pages, 8 figures, 2 tables.-- Printed version published Nov 2003.
An expression system based on Escherichia coli and the T5 promoter allowed the overproduction of a his-tagged rhamnulose-1-phosphate aldolase (RhuA; EC 4.1.2.19), an enzyme with applications in the production of deoxyazasugars and deoxysugars compounds. Shake flask and bioreactor cultivation with E coli M15 (pQErham) were performed under different media and inducing conditions for RhuA expression. A Defined Medium (DM) with glucose as carbon source gave a high volumetric and enzyme productivity (3460 AU dm-3 and 288 AU dm-3 h-1 respectively) compared with Luria-Bertoni (LB) medium (2292 AU dm- 3 and 255 AU dm-3 h-1). The minimum quantity of (isopropyl--D-thiogalactoside) IPTG for optimal induction was estimated in 18-20 ºmol IPTG gDCW-1. The highest volumetric production of RhuA (8333 AU dm-3) was obtained when IPTG was added in the late log-phase. No significant differences were found in specific RhuA activity for induction temperatures of 30 and 37 ºC. An effective two-step purification process comprising affinity chromatography and gel permeation has been developed (overall recovery 66.5%). These studies provide the basis for the further development of an integrated process for recombinant RhuA production suitable for biotransformation applications.
2010-03-17T09:16:48Z
2010-03-17T09:16:48Z
2003-09-18
artículo
Journal of Chemical Technology and Biotechnology 78(11): 1171-1179 (2003)
0268-2575
http://hdl.handle.net/10261/22452
10.1002/jctb.909
1097-4660
eng
http://dx.doi.org/10.1002/jctb.909
closedAccess
Wiley-Blackwell