2024-03-28T20:59:54Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/2208952021-12-27T16:41:49Zcom_10261_175539com_10261_1col_10261_175590
2020-10-07T08:58:51Z
urn:hdl:10261/220895
Isolation of four lytic phages infecting Klebsiella pneumoniae K22 clinical isolates from Spain
Domingo-Calap, Pilar
Beamud, Beatriz
Vienne, Justine
González-Candelas, Fernando
Sanjuán, Rafael
European Commission
European Research Council
Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
Generalitat Valenciana
Klebsiella pneumoniae
Phage therapy
Bacteriophage
This article belongs to the Special Issue Bacteriophage—Molecular Studies.
The emergence of multi-drug-resistant bacteria represents a major public-health threat. Phages constitute a promising alternative to chemical antibiotics due to their high host specificity, abundance in nature, and evolvability. However, phage host specificity means that highly diverse bacterial species are particularly difficult to target for phage therapy. This is the case of Klebsiella pneumoniae, which presents a hypervariable extracellular matrix capsule exhibiting dozens of variants. Here, we report four novel phages infecting K. pneumoniae capsular type K22 which were isolated from environmental samples in Valencia, Spain. Full genome sequencing showed that these phages belong to the Podoviridae family and encode putative depolymerases that allow digestion of specific K22 K. pneumoniae capsules. Our results confirm the capsular type-specificity of K. pneumoniae phages, as indicated by their narrow infectivity in a panel of K. pneumoniae clinical isolates. Nonetheless, this work represents a step forward in the characterization of phage diversity, which may culminate in the future use of large panels of phages for typing and/or for combating multi-drug-resistant K. pneumoniae.
2020-10-07T08:58:51Z
2020-10-07T08:58:51Z
2020
artículo
International Journal of Molecular Sciences 21(2): 425 (2020)
http://hdl.handle.net/10261/220895
10.3390/ijms21020425
1422-0067
http://dx.doi.org/10.13039/501100011033
http://dx.doi.org/10.13039/501100003359
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100000781
31936552
eng
Publisher's version
https://doi.org/10.3390/ijms21020425
Sí
info:eu-repo/grantAgreement/EC/H2020/724519
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/BFU2017-89594-R
BFU2017-89594-R/AEI/10.13039/501100011033
https://creativecommons.org/licenses/by/4.0/
openAccess
Multidisciplinary Digital Publishing Institute