2024-03-29T12:32:37Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/202342018-08-30T07:06:10Zcom_10261_63com_10261_6col_10261_442
2010-01-21T11:25:36Z
urn:hdl:10261/20234
An exogenous protease increases enzymic activities, microbial numbers and fiber degraation by mixed ruminal microorganisms in continuous culture
Colombatto, Darío
Hervás, Gonzalo
Beauchemin, Karen A.
Contributed to: Conference on Gastrointestinal Function (Chicago, Illinois, Mar 10-12, 2003).
The effects of pH and addition of a protease mixture on the fermentation characteristics of a total
mixed ration (TMR) were investigated in a dual-flow continuous culture apparatus, using a 4 x 4
Latin Square design with a 2 x 2 factorial arrangement of treatments. The diet (DM basis)
consisted of 30% alfalfa hay, 30% corn silage and 40% rolled corn. The silage and the grain
were milled fresh, mixed with the alfalfa and treated with the enzyme (1.5 μL/g feed) daily.
Ruminal fluid was collected 2 h post-feeding from 3 lactating dairy cows fed a TMR. Fermenters
were fed 80 g DM/d in equal portions every 12 h. Treatments were control (C) and enzymetreated
(T) TMR at either high pH (HC and HT) or low pH (LC and LT). The pH was altered by
diluting the artificial saliva to 60% of its original composition. Enzymic activities and total and
cellulolytic bacterial numbers were determined on the liquid phase of the fermenter contents, 6 h
post-feeding. Fiber degradation was determined from the outflow residues. Enzyme addition
increased (P < 0.05) xylanase, xylosidase, endoglucanase, and protease activities (608 vs. 750;
0.48 vs. 0.80; 82 vs. 112; and 1.2 vs. 7.5 units for C and T, respectively), whereas it tended (P <
0.12) to increase exoglucanase and glucosidase activities (0.8 vs. 1.4; and 4.7 vs. 5.9 units).
However, enzyme did not affect (P = 0.18) arabinofuranosidase activity (5.2 vs. 6.8). Total
microbial numbers (expressed as Log10) were increased (P < 0.05) at low pH (9.13 vs. 9.36) but
enzyme had no effect (P = 0.13). Cellulolytic bacteria were reduced (P < 0.02) at low pH (3.91
vs. 2.79), with no effect (P = 0.88) of enzyme. Low pH reduced (P < 0.001) NDF, ADF, and
cellulose degradation. NDF degradation was increased (P < 0.01) by enzyme addition (20% vs.
27%) but ADF was unaffected (P < 0.20), resulting in an increase (P < 0.001) in hemicellulose
degradation. It is speculated that the enzyme removed structural barriers present in the feed,
allowing a more rapid colonization of the fiber by ruminal microorganisms.
2010-01-21T11:25:36Z
2010-01-21T11:25:36Z
2003
comunicación de congreso
Conference on Gastrointestinal Function, p. 17 (2003)
http://hdl.handle.net/10261/20234
eng
openAccess