2024-03-28T20:43:27Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/2015062021-12-27T15:39:32Zcom_10261_109com_10261_1col_10261_362
2020-02-21T10:43:09Z
urn:hdl:10261/201506
Tuberculosis in Liberia: high multidrug-resistance burden, transmission and diversity modelled by multiple importation events
López, Mariana G.
Dogba, John B.
Torres-Puente, Manuela
Goig, Galo A.
Moreno-Molina, Miguel
Villamayor, Luis M.
Cadmus, Simeon
Comas, Iñaki
European Research Council
Ministerio de Economía y Competitividad (España)
John D. and Catherine T. MacArthur Foundation
Comas, Iñaki [0000-0001-5504-9408]
Torres-Puente, Manuela [0000-0002-8352-180X]
López, Mariana G. [0000-0002-2216-9232]
Liberia
TB transmission
WGS
Drug resistances
Tuberculosis
Whole-genome sequencing
12 páginas, 5 figuras, 2 tablas. DNA sequences generated as part of this study have been deposited at the European Nucleotide Archive under study accession number PRJEB32589 (Table S1) (2019)
Tuberculosis (TB) surveillance is scarce in most African countries, even though it is the continent with the greatest disease incidence according to the World Health Organization. Liberia is within the 30 countries with the highest TB burden, probably as a consequence of the long civil war and the recent Ebola outbreak, both crippling the health system and depreciating the TB prevention and control programmes. Due to difficulties working in the country, there is a lack of resistance surveys and bacillus characterization. Here, we use genome sequencing of Mycobacterium tuberculosis clinical isolates to fill this gap. Our results highlight that the bacillus population structure is dominated by lineage 4 strains that harbour an outstanding genetic diversity, higher than in the rest of Africa as a whole. Coalescent analyses demonstrate that strains currently circulating in Liberia were introduced several times beginning in the early year 600 CE until very recently coinciding with migratory movements associated with the civil war and Ebola epidemics. A higher multidrug-resistant (MDR)-TB frequency (23.5 %) than current estimates was obtained together with non-catalogued drug-resistance mutations. Additionally, 39 % of strains were in genomic clusters revealing that ongoing transmission is a major contribution to the TB burden in the country. Our report emphasizes the importance of TB surveillance and control in African countries where bacillus diversity, MDR-TB prevalence and transmission are coalescing to jeopardize TB control programmes.
2020-02-21T10:43:09Z
2020-02-21T10:43:09Z
2020-01-01
artículo
Microbial Genomics 6(1) (2020)
http://hdl.handle.net/10261/201506
10.1099/mgen.0.000325
2057-5858
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100000781
31935183
eng
Publisher's version
http://dx.doi.org/10.1099/mgen.0.000325
Sí
info:eu-repo/grantAgreement/EC/H2020/638553
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SAF2013-43521-R
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/SAF2016-77346-R
http://creativecommons.org/licenses/by/4.0/
openAccess
Microbiology Society