2024-03-29T13:39:35Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/196092018-09-11T10:23:45Zcom_10261_15com_10261_6col_10261_268
2009-12-16T10:35:13Z
urn:hdl:10261/19609
CRISPR elements in the thermococcales: evidence for associated
Portillo Guisado, María del Carmen
González Grau, Juan Miguel
Junta de Andalucía
CRISPR
Gene transfer
Genome
Hyperthermophilic Archaea
Pyrococcus furiosus
10 pages, 2 figures, 2 tables.
The presence and distribution of CRISPR (clustered regularly interspaced short palindrome repeat) elements in the archaeal order Thermococcales were analyzed. Four complete genome sequences from the species Pyrococcus abyssi, P. furiosus, P. horikoshii, and Thermococcus kodakaraensis were studied. A fragment of the genome of P. furiosus was flanked by CRISPR elements upstream and by a single element downstream. The composition of the gene sequences contained in this genome fragment (positions 699013 to 855319) showed significant differences from the other genes in the P. furiosus genome. Differences were observed in the GC content at the third codon positions and the frequency of codon usage between the genes located in the analyzed fragment and the other genes in the P. furiosus genome. These results represent the first evidence suggesting that repeated CRISPR elements can be involved in horizontal gene transfer and genomic differentiation of hyperthermophilic Archaea.
2009-12-16T10:35:13Z
2009-12-16T10:35:13Z
2009
artículo
Journal of Applied Genetics 50(4): 421-430 (2009)
1234-1983
http://hdl.handle.net/10261/19609
http://dx.doi.org/10.13039/501100011011
eng
http://jag.igr.poznan.pl/2009-Volume-50/4/abstracts/538.html
openAccess
Institute of Plant Genetics
Polish Academy of Sciences