2024-03-29T07:14:09Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1802422019-04-18T00:57:44Zcom_10261_134com_10261_1col_10261_387
2019-04-17T09:30:36Z
urn:hdl:10261/180242
Role of the Wilms' tumor suppressor gene Wt1 in pancreatic development
Ariza, Laura
Cañete, Ana
Rojas, Anabel
Muñoz-Chápuli, Ramón
Carmona, Rita
Ministerio de Economía y Competitividad (España)
Instituto de Salud Carlos III
Red de Terapia Celular (España)
Junta de Andalucía
Carmona, Rita [0000-0002-9686-473X]
Mesothelium
Epithelial–mesenchymal transition
Pancreatic stellate cells
Pancreatic stroma
Intestinal rotation
The Wilms tumor suppressor gene (Wt1) encodes a transcription factor involved in the development of a number of organs, but the role played by Wt1 in pancreatic development is unknown. The pancreas contains a population of pancreatic stellate cells (PSC) very important for pancreatic physiology. We described elsewhere that hepatic stellate cells originate from the WT1‐expressing liver mesothelium. Thus, we checked if the origin of PSCs was similar. WT1 expression is restricted to the pancreatic mesothelium. Between embryonic day (E) 10.5 and E15.5, this mesothelium gives rise to mesenchymal cells that contribute to a major part of the PSC and other cell types including endothelial cells. Most WT1 systemic mutants show abnormal localization of the dorsal pancreas within the mesentery and intestinal malrotation by E14.0. Embryos with conditional deletion of WT1 between E9.5 and E12.5 showed normal dorsal pancreatic bud and intestine, but the number of acini in the ventral bud was reduced approximately 30% by E16.5. Proliferation of acinar cells was reduced in WT1 systemic mutants, but pancreatic differentiation was not impaired. Thus, mesothelial‐derived cells constitute an important subpopulation of pancreatic mesodermal cells. WT1 expression is not essential for pancreas development, although it influences intestinal rotation and correct localization of the dorsal pancreas within the mesogastrium.
2019-04-17T09:30:36Z
2019-04-17T09:30:36Z
2018-07
artículo
Developmental Dynamics 247(7): 924-933 (2018)
1058-8388
http://hdl.handle.net/10261/180242
10.1002/dvdy.24636
1097-0177
http://dx.doi.org/10.13039/501100004587
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100011011
eng
https://doi.org/10.1002/dvdy.24636
Sí
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2014‐52299‐P
closedAccess
John Wiley & Sons