2024-03-28T13:29:54Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1710052021-11-22T13:08:20Zcom_10261_59com_10261_6col_10261_312
2018-10-15T07:41:41Z
urn:hdl:10261/171005
FleQ coordinates flagellum-dependent and -independent motilities in Pseudomonas syringae pv. tomato DC3000
Nogales, Joaquina
Vargas, P.
Farias, Gabriela A.
Olmedilla, Adela
Sanjuán, Juan
Gallegos, María Trinidad
Ministerio de Economía y Competitividad (España)
Junta de Andalucía
European Commission
Ministério da Educação (Brasil)
© 2015, American Society for Microbiology. Motility plays an essential role in bacterial fitness and colonization in the plant environment, since it favors nutrient acquisition and avoidance of toxic substances, successful competition with other microorganisms, the ability to locate the preferred hosts, access to optimal sites within them, and dispersal in the environment during the course of transmission. In this work, we have observed that the mutation of the flagellar master regulatory gene, fleQ, alters bacterial surface motility and biosurfactant production, uncovering a new type of motility for Pseudomonas syringae pv. tomato DC3000 on semisolid surfaces. We present evidence that P. syringae pv. tomato DC3000 moves over semisolid surfaces by using at least two different types of motility, namely, swarming, which depends on the presence of flagella and syringafactin, a biosurfactant produced by this strain, and a flagellumindependent surface spreading or sliding, which also requires syringafactin. We also show that FleQ activates flagellum synthesis and negatively regulates syringafactin production in P. syringae pv. tomato DC3000. Finally, it was surprising to observe that mutants lacking flagella or syringafactin were as virulent as the wild type, and only the simultaneous loss of both flagella and syringafactin impairs the ability of P. syringae pv. tomato DC3000 to colonize tomato host plants and cause disease.
2018-10-15T07:41:41Z
2018-10-15T07:41:41Z
2015
2018-10-15T07:41:42Z
artículo
Applied and Environmental Microbiology 81: 7533- 7545 (2015)
http://hdl.handle.net/10261/171005
10.1128/AEM.01798-15
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100006366
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100011011
26296726
eng
Sí
closedAccess
American Society for Microbiology