2024-03-30T06:01:32Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1680112020-11-13T12:55:45Zcom_10261_86com_10261_1col_10261_339
2018-07-27T11:56:42Z
urn:hdl:10261/168011
MalR-mediated regulation of the Streptococcus pneumoniae malMP operon at promoter PM. Influence of a proximal divergent promoter region and competition between MalR and RNA polymerase proteins
Nieto, Concha
Puyet, A.
Espinosa, Manuel
Comisión Interministerial de Ciencia y Tecnología, CICYT (España)
Comunidad de Madrid
Puyet, A. [0000-0002-2026-7955]
Espinosa, Manuel [0000-0003-3958-0543]
Staphylococcus-aureus
Escherichia-coli
Catabolite repression
Bacillus-subtilis
Open-complex
Transcription
DNA
Expression
Site
Inhibition
10 p.-7 fig.-1 tab.
The Streptococcus pneumoniae malregulon contains two operons, malXCD andmalMP involved in the uptake and utilization of maltosaccharides. Both operons are transcribed from two divergent promoters, PX andPM , and are negatively regulated by the MalR transcriptional repressor. Purified MalR protein binds to two DNA regions that encompasses both promoters, thus occupying its two operators, OM and OX. However, the levels of occupation and repression were different, being higher when MalR was bound to OM than when it was anchored to OX. Competition experiments between MalR and the Escherichia coli RNA polymerase on promoters PM andPX showed that the affinity of either protein for the promoter/operator DNA sequences was important to determine the frequency of transcription initiation. In addition to the control exerted by MalR, expression from promoter PM was affected by upstream sequences located within or close toPX promoter.
2018-07-27T11:56:42Z
2018-07-27T11:56:42Z
2001-05-04
artículo
The Journal of Biological Chemistry 276, 14946-14954 (2001)
0021-9258
http://hdl.handle.net/10261/168011
10.1074/jbc.M010911200
1083-351X
http://dx.doi.org/10.13039/501100007273
http://dx.doi.org/10.13039/100012818
eng
Publisher's version
https://doi.org/10.1074/jbc.M010911200
Sí
openAccess
American Society for Biochemistry and Molecular Biology