2024-03-28T11:56:58Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1649742020-12-13T09:16:29Zcom_10261_79com_10261_1col_10261_332
2018-05-22T11:13:49Z
urn:hdl:10261/164974
Adenohypophysis placodal precursors exhibit distinctive features within the rostral preplacodal ectoderm
Sánchez-Arrones, Luisa
Sandonís Consuegra, África
Cardozo, Marcos
Bovolenta, Paola
Ministerio de Economía y Competitividad (España)
Centro de Investigación Biomédica en Red Enfermedades Raras (España)
Instituto de Salud Carlos III
Fundación Ramón Areces
Comunidad de Madrid
RNA-seq
Chicks
Pituitary
Midline
Cell movement
Adhesion
Sp8
Placodes are discrete thickenings of the vertebrate cranial ectoderm that generate morpho-functionally distinct structures, such as the adenohypophysis, olfactory epithelium and lens. All placodes arise from a horseshoe-shaped preplacodal ectoderm in which the precursors of individual placodes are intermingled. However, fatemap studies indicated that cells positioned at the preplacodal midline give rise to only the adenohypophyseal placode, suggesting a unique organization of these precursors within the preplacode. To test this possibility, we combined embryological and molecular approaches in chick embryos to show that, at gastrula stage, adenohypophyseal precursors are clustered in the median preplacodal ectoderm, largely segregated from those of the adjacent olfactory placode. Median precursors are elongated, densely packed and, at neurula stage, express a molecular signature that distinguishes them from the remaining preplacodal cells. Olfactory placode precursors and midline neural cells can replace ablated adenohypophyseal precursors up to head-fold stage, although with a more plastic organization. We thus propose that adenohypophyseal placode precursors are unique within the preplacodal ectoderm possibly because they originate the only single placode and the only one with an endocrine character.
2018-05-22T11:13:49Z
2018-05-22T11:13:49Z
2017
2018-05-22T11:13:50Z
artículo
Development 144: 3521- 3532 (2017)
http://hdl.handle.net/10261/164974
10.1242/dev.149724
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100004587
http://dx.doi.org/10.13039/100008054
http://dx.doi.org/10.13039/100012818
eng
Publisher's version
Sí
openAccess
Company of Biologists