2024-03-28T22:25:24Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1631682020-12-10T17:15:59Zcom_10261_128com_10261_1col_10261_381
2018-04-05T09:00:10Z
urn:hdl:10261/163168
A Pseudomonas putida cbrB transposon insertion mutant displays a biofilm hyperproducing phenotype that is resistant to dispersal
Amador, Cristina I.
López-Sánchez, Aroa
Govantes, Fernando
Santero, Eduardo
Canosa, Inés
Junta de Andalucía
Ministerio de Economía y Competitividad (España)
Ministerio de Ciencia e Innovación (España)
Biofilms
Pseudomonas putida
CbrB
Environmental genomics
The CbrAB two-component system in the Pseudomonads controls a variety of metabolic and behavioural traits required for its adaptation to changing environmental conditions, including the uptake or assimilation of certain carbon sources, and processes such as chemotaxis or stress tolerance. In this work we characterise a miniTn5-luxAB-Km transposon insertion mutant in cbrB (MPO406) in Pseudomonas putida leading to a biofilm overproducing phenotype that is not dispersed when nutrients are depleted. Comparison with a cbrB deletion mutant revealed that all phenotypes previously attributed to CbrB in P. putida correlated in both strains, with the exception of biofilm overproduction and absence of dispersal. We show that in the insertion mutant, the expression of the downstream regulatory RNA CrcZ is upregulated, and also show the presence of a truncated form of CbrB. Also, two additional point mutations in lapG and lapD have been detected in MPO406 by whole genome sequencing. Combination of these effects provides a robust biofilm overproducing phenotype. We present the mutant strain MPO406 as a good candidate to perform bio-production of substances of biotechnological interest or other processes such as bioremediation, which take advantage of immobilized cells on solid surfaces.
2018-04-05T09:00:10Z
2018-04-05T09:00:10Z
2016
2018-04-05T09:00:10Z
artículo
Environmental Microbiology Reports 8(5): 622-629 (2016)
http://hdl.handle.net/10261/163168
10.1111/1758-2229.12414
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100004837
http://dx.doi.org/10.13039/501100011011
27085034
eng
Preprint
https://doi.org/10.1111/1758-2229.12414
Sí
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2014-57545-R
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2013-42073-P
openAccess
Wiley-VCH
Society for Applied Microbiology