2024-03-29T07:15:49Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1620982021-03-18T16:13:22Zcom_10261_63com_10261_6col_10261_316
2018-03-13T09:25:39Z
urn:hdl:10261/162098
Synthesis of the suspected trans-11,cis-13 conjugated linoleic acid isomer in ruminant mammary tissue by FADS3-catalyzed Δ13-desaturation of vaccenic acid
García, C.
Duby, C.
Catheline, D.
Toral, Pablo G.
Bernard, Laurence
Legrand, P.
Rioux, V.
Institut National de la Recherche Agronomique (France)
Agence Nationale de la Recherche (France)
cis-13 conjugated
linoleic acid
Fatty acid desaturase 3, Stearoyl-CoA
desaturase 1
trans-11
Vaccenic acid
14 páginas, 7 figuras, 1 tabla.
The octadecadienoic conjugated linoleic acid (CLA)
isomer with trans-11 and cis-13 double bonds (trans11,cis-13
CLA) has been described in ruminant milk.
For now, this specific CLA is suspected to derive exclusively
from ruminal biohydrogenation of dietary
α-linolenic acid. However, in rodents, the fatty acid
desaturase 3 (FADS3) gene was recently shown to code
for an enzyme able to catalyze the unexpected Δ13-
desaturation of vaccenic acid, producing a Δ11,13-CLA
with all the structural characteristics of the trans11,cis-13
isomer, although no commercial standard
exists for complete conclusive identification. Because
the FADS3 gene has already been reported in bovine
animals, we hypothesized in the present study that an
alternative direct FADS3-catalyzed Δ13-desaturation
of vaccenic acid in mammary tissue may therefore coexist
with α-linolenic acid biohydrogenation to explain
the final ruminant milk trans-11,cis-13 CLA presence.
Here, we first confirm that the FADS3 gene is present
in ruminant mammal genomic sequence databases.
Second, we demonstrate that the Δ11,13-CLA found in
milk fat and the highly probable trans-11,cis-13 CLA
isomer produced by rodent FADS3 possess exactly the
same structural characteristics. Then, we show that
bovine mammary MAC-T and BME-UV epithelial cells
express both FADS3 and stearoyl-CoA desaturase 1
(SCD1) mRNA and are able to synthesize both the
suspected trans-11,cis-13 CLA and cis-9,trans-11CLA
(rumenic acid) isomers when incubated with vaccenic
acid. Finally, the concomitant presence of the suspected
trans-11,cis-13 CLA isomer with FADS3 mRNA was
shown in goat mammary tissue, whereas both were conversely
very low or even absent in goat liver. Therefore,
this study provides several lines of evidence that, by
analogy with rumenic acid, trans-11,cis-13 CLA may
originate both from ruminal biohydrogenation and
from direct FADS3-catalyzed Δ13-desaturation of vaccenic
acid in mammary tissue.
2018-03-13T09:25:39Z
2018-03-13T09:25:39Z
2017
2018-03-13T09:25:39Z
artículo
Journal of Dairy Science 100: 783- 796 (2017)
0022-0302
http://hdl.handle.net/10261/162098
10.3168/jds.2016-11455
http://dx.doi.org/10.13039/501100006488
http://dx.doi.org/10.13039/501100001665
eng
http://dx.doi.org/10.3168/jds.2016-11455
Sí
closedAccess
American Dairy Science Association