2024-03-29T15:01:14Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1569972018-10-03T07:01:44Zcom_10261_9676com_10261_8col_10261_9679
2017-11-06T09:36:20Z
urn:hdl:10261/156997
Exploring the phenotypic space of a putatively adaptive transposable element insertion in Drosophila
Ullastres, Ana
González Pérez, Josefa
Trabajo presentado en la XIV Jornada de Biología Evolutiva, celebrada en Barcelona el 1 de julio de 2014.
The ínvader4 retro-transposon FBtí0019386 was identified as a putatively adaptive
natural insertion in a genome-wide screening in Drosophíla melanogaster. In this study,
we screened multiple phenotypes in several D. melanogaster natural populations
differing by the presence/absence of this transposable element (TE) to identify the
adaptive effect of this mutation. To do this, we took into account FBtí0019386
population frequency dynamics suggesting that it is involved in adaptation to temperate
environments as well as the functional information available for the two closest genes.
FBtí0019386 is inserted in the 5'UTR intron of sarah, a gene involved in a wide variety
of processes such as ovulation, egg hatchability, fecundity or learning and memory.
FBtí0019386 is located upstream of the 5'UTR of bicoid interacting protein 1 (Bin1), a
negative regulator of transcription that plays a role in environmental stress response.
We found that flies with and without the insertion do not differ in fecundity or egg
hatchability. However, we observed that flies with the insertion are more sensitive to
cold stress in adults, and slightly more sensitive to osmotic stress and starvation, while
they do not differ in cold stress sensitivity in embryos. Our results also showed that
flies with the insertion have a significantly shorter developmental time compared to flies
without the insertion. The observed increased sensitivity to environmental stress is
likely to be the cost of selection of this adaptive mutation, while a shorter
developmental time is an adaptive fitness trait in nature that is associated with
temperate environments adaptation. We also found that flies with FBtí0019386
insertion showed a down-regulation of sarah but not of Bin1 . This down-regulation is
most probably dueto the introduction of piRNA-binding sites by FBti00193861eading to
the recruitment of HP1 protein.
While we cannot discard that other phenotypes such as fine-tuning regulation of
learning and memory could also be affected by FBtí0019386, our results already show
that TE-induced mutations have complex phenotypic effects with both positive and
negative impacts on fitness.
2017-11-06T09:36:20Z
2017-11-06T09:36:20Z
2017-07
comunicación de congreso
XIV Jornada de Biología Evolutiva (2014)
http://hdl.handle.net/10261/156997
eng
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