2024-03-28T16:20:51Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1530452021-12-28T16:02:00Zcom_10261_74com_10261_6col_10261_327
2017-07-14T12:02:03Z
urn:hdl:10261/153045
Target and Non-target Site Mechanisms Developed by Glyphosate-Resistant Hairy beggarticks (Bidens pilosa L.) Populations from Mexico
Alcántara de la Cruz, Ricardo
Fernández-Moreno, Pablo T.
Ozuna, Carmen
Rojano-Delgado, Antonia María
Cruz-Hipólito, Hugo Enrique
Domínguez Valenzuela, José A.
Barro Losada, Francisco
Prado, R. del
Comisión Nacional de Investigación Científica y Tecnológica (Chile)
In 2014 hairy beggarticks (Bidens pilosa L.) has been identified as being glyphosate-resistant in citrus orchards from Mexico. The target and non-target site mechanisms involved in the response to glyphosate of two resistant populations (R1 and R2) and one susceptible (S) were studied. Experiments of dose-response, shikimic acid accumulation, uptake-translocation, enzyme activity and 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) gene sequencing were carried out in each population. The R1 and R2 populations were 20.4 and 2.8-fold less glyphosate sensitive, respectively, than the S population. The resistant populations showed a lesser shikimic acid accumulation than the S population. In the latter one, 24.9% of 14C-glyphosate was translocated to the roots at 96 h after treatment; in the R1 and R2 populations only 12.9 and 15.5%, respectively, was translocated. Qualitative results confirmed the reduced 14C-glyphosate translocation in the resistant populations. The EPSPS enzyme activity of the S population was 128.4 and 8.5-fold higher than the R1 and R2 populations of glyphosate-treated plants, respectively. A single (Pro-106-Ser), and a double (Thr-102-Ile followed by Pro-106-Ser) mutations were identified in the EPSPS2 gene conferred high resistance in R1 population. Target-site mutations associated with a reduced translocation were responsible for the higher glyphosate resistance in the R1 population. The low-intermediate resistance of the R2 population was mediated by reduced translocation. This is the first glyphosate resistance case confirmed in hairy beggarticks in the world.
2017-07-14T12:02:03Z
2017-07-14T12:02:03Z
2016-10-03
2017-07-14T12:02:03Z
artículo
Frontiers in Plant Science 7: 1492 (2016)
1664-462X
http://hdl.handle.net/10261/153045
10.3389/fpls.2016.01492
http://dx.doi.org/10.13039/501100002848
27752259
en
Publisher's version
http://dx.doi.org/10.3389/fpls.2016.01492
Sí
Copyright © 2016 Alcántara-de la Cruz, Fernández-Moreno, Ozuna, Rojano-Delgado, Cruz-Hipolito, Domínguez-Valenzuela, Barro and De Prado.
http://creativecommons.org/licenses/by/4.0/
openAccess
Frontiers Media