2024-03-29T08:07:37Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1519752021-12-28T16:35:57Zcom_10261_80com_10261_1col_10261_333
2017-06-25T03:21:18Z
urn:hdl:10261/151975
Management of enzyme diversity in high-performance cellulolytic cocktails
Reyes-Sosa, Francisco M.
López Morales, Macarena
Platero Gómez, Ana Isabel
Valbuena Crespo, Noelia
Sánchez Zamorano, Laura
Rocha-Martín, Javier
Molina-Heredia, Fernando P.
Diéz-García, Bruno
European Commission
Ministerio de Economía y Competitividad (España)
Enzymatic hydrolysis
Cellulolytic cocktail improvement
Lignocellulosic biomass
Bioethanol
[Background]
Modern biorefineries require enzymatic cocktails of improved efficiency to generate fermentable sugars from lignocellulosic biomass. Cellulolytic fungi, among other microorganisms, have demonstrated the highest potential in terms of enzymatic productivity, complexity and efficiency. On the other hand, under cellulolytic-inducing conditions, they often produce a considerable diversity of carbohydrate-active enzymes which allow them to adapt to changing environmental conditions. However, industrial conditions are fixed and adjusted to the optimum of the whole cocktail, resulting in underperformance of individual enzymes.
[Results]
One of these cellulolytic cocktails from Myceliophthora thermophila has been analyzed here by means of LC–MS/MS. Pure GH6 family members detected have been characterized, confirming previous studies, and added to whole cocktails to compare their contribution in the hydrolysis of industrial substrates. Finally, independent deletions of two GH6 family members, as an example of the enzymatic diversity management, led to the development of a strain producing a more efficient cellulolytic cocktail.
[Conclusions]
These data indicate that the deletion of noncontributive cellulases (here EG VI) can increase the cellulolytic efficiency of the cocktail, validating the management of cellulase diversity as a strategy to obtain improved fungal cellulolytic cocktails.
2017-06-25T03:21:18Z
2017-06-25T03:21:18Z
2017-06-19
2017-06-25T03:21:19Z
artículo
Biotechnology for Biofuels 10(1): 156 (2017)
1754-6834
http://hdl.handle.net/10261/151975
10.1186/s13068-017-0845-6
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100003329
28649275
en
Publisher's version
http://dx.doi.org/10.1186/s13068-017-0845-6
Sí
info:eu-repo/grantAgreement/EC/FP7/604530
The Author(s)
http://creativecommons.org/licenses/by/4.0/
openAccess
BioMed Central