2024-03-28T22:12:39Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1505402021-12-28T16:24:15Zcom_10261_31891com_10261_2col_10261_31892
2017-05-29T07:58:08Z
urn:hdl:10261/150540
Nano-liquid chromatography-orbitrap ms-based quantitative proteomics reveals differences between the mechanisms of action of carnosic acid and carnosol in colon cancer cells
Valdés, Alberto
García-Cañas, Virginia
Artemenko, Konstantin A.
Simó, Carolina
Bergquist, Jonas
Cifuentes, Alejandro
Ministerio de Economía y Competitividad (España)
Comunidad de Madrid
Swedish Research Council
Cancer biology
Carnosic acid
HT-29 cells
Foodomics
Dimethyl labeling
Carnosol
Colorectal cancer
Mass spectrometry
Proliferation
Quantification
Carnosic acid (CA) and carnosol (CS) are two structurally related diterpenes present in rosemary herb (Rosmarinus officinalis). Although several studies have demonstrated that both diterpenes can scavenge free radicals and interfere in cellular processes such as cell proliferation, they may not necessarily exert the same effects at the molecular level. In this work, a shotgun proteomics study based on stable isotope dimethyl labeling (DML) and nano-liquid chromatography-tandem mass spectrometry (nano-LC-MS/MS) has been performed to identify the relative changes in proteins and to gain some light on the specific molecular targets and mechanisms of action of CA and CS in HT-29 colon cancer cells. Protein profiles revealed that CA and CS induce different Nrf2-mediated response. Furthermore, examination of our data revealed that each diterpene affects protein homeostasis by different mechanisms. CA treatment induces the expression of proteins involved in the unfolded protein response in a concentration dependent manner reflecting ER stress, whereas CS directly inhibits chymotrypsin-like activity of the 20S proteasome. In conclusion, the unbiased proteomics-wide method applied in the present study has demonstrated to be a powerful tool to reveal differences on the mechanisms of action of two related bioactive compounds in the same biological model.
2017-05-29T07:58:08Z
2017-05-29T07:58:08Z
2017
2017-05-29T07:58:09Z
artículo
Molecular and Cellular Proteomics 16(1): 8-22 (2017)
http://hdl.handle.net/10261/150540
10.1074/mcp.M116.061481
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/100012818
27834734
eng
Postprint
https://doi.org/10.1074/mcp.M116.061481
Sí
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2014-53609-P
S2013/ABI-2728/ALIBIRD-CM
openAccess
American Society for Biochemistry and Molecular Biology