2024-03-29T09:55:00Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1489982021-12-27T16:02:41Zcom_10261_75com_10261_6col_10261_328
2017-05-02T11:07:26Z
urn:hdl:10261/148998
Large differences in gene expression responses to drought and heat stress between elite barley cultivar scarlett and a spanish landrace
Pérez Cantalapiedra, Carlos
García-Pereira, María J.
Gracia Gimeno, María Pilar
Igartua Arregui, Ernesto
Casas Cendoya, Ana María
Contreras-Moreira, Bruno
Gobierno de Aragón
La Caixa
Ministerio de Economía y Competitividad (España)
Barley
landrace
drought
heat
transcriptome profiling
gene expression
RNAseq
23 Pags.- 6 Tabls.- 8 Figs. Copyright © 2017 Cantalapiedra, García-Pereira, Gracia, Igartua, Casas and Contreras-Moreira. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forms is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Drought causes important losses in crop production every season. Improvement for
drought tolerance could take advantage of the diversity held in germplasm collections,
much of which has not been incorporated yet into modern breeding. Spanish landraces
constitute a promising resource for barley breeding, as they were widely grown until last
century and still show good yielding ability under stress. Here, we study the transcriptome
expression landscape in two genotypes, an outstanding Spanish landrace-derived inbred
line (SBCC073) and a modern cultivar (Scarlett). Gene expression of adult plants after
prolonged stresses, either drought or drought combined with heat, was monitored.
Transcriptome of mature leaves presented little changes under severe drought, whereas
abundant gene expression changes were observed under combined mild drought
and heat. Developing inflorescences of SBCC073 exhibited mostly unaltered gene
expression, whereas numerous changes were found in the same tissues for Scarlett.
Genotypic differences in physiological traits and gene expression patterns confirmed
the different behavior of landrace SBCC073 and cultivar Scarlett under abiotic stress,
suggesting that they responded to stress following different strategies. A comparison
with related studies in barley, addressing gene expression responses to drought, revealed
common biological processes, but moderate agreement regarding individual differentially
expressed transcripts. Special emphasis was put in the search of co-expressed
genes and underlying common regulatory motifs. Overall, 11 transcription factors were
identified, and one of them matched cis-regulatory motifs discovered upstream of
co-expressed genes involved in those responses.
2017-05-02T11:07:26Z
2017-05-02T11:07:26Z
2017-05
artículo
Cantalapiedra CP, García-Pereira MJ, Gracia MP, Igartua E, Casas AM, Contreras-Moreira B. Large differences in gene expression responses to drought and heat stress between elite barley cultivar scarlett and a spanish landrace. Frontiers in Plant Science 8: 647, doi: 10.3389/fpls.2017.00647 (2017)
http://hdl.handle.net/10261/148998
10.3389/fpls.2017.00647
1664-462X
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100010067
28507554
eng
Publisher's version
Sí
https://creativecommons.org/licenses/by-nc-nd/4.0/
openAccess
Frontiers Media