2024-03-19T02:15:11Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1470252017-03-22T01:53:36Zcom_10261_10735com_10261_2col_10261_10737
2017-03-21T10:03:12Z
urn:hdl:10261/147025
In the microvine pericarp, critical transcriptome changes occur during the night
Rienth, Markus
Torregrosa, Laurent
Kelly, Mary T.
Luchaire, Nathalie
Grimplet, Jérôme
Romieu, Charles
Póster presentado en la 2nd Annual ConferenceFleshy Fruit Development & Ripening (COST Action FA1106 QualityFruit), celebrada en Chania (Grecia) del 22 al 25 de septiembre de 2013.
Many transcriptomic studies have been conducted on
different
vitis vinifera
L. cultivars to
better understand physiological changes occurring d
uring berry development (Lijavetzky et
al., 2012, Terrier et al., 2005, Pilati et al., 200
7). To our knowledge very few time course
studies, assessing both day and night transcriptome
, have been performed on any fleshy
fruits until now. Still, it was demonstrated on mod
el plants such as Arabidopsis thaliana
(Schaffer et al., 2001, Chow and Kayb, 2013) that i
mportant changes in gene expression
following diurnal patterns occur in all living orga
nisms and might influence organoleptic
quality of the grapes. With the macrovine, which pr
esents an annual reproductive cycle,
studying day and night fruit development under cont
rolled conditions is almost impossible.
In our study, we useed the microvine (DRCF-mutant;
Boss and Thomas, 2002) a recently
proposed grapevine model (Chaib et al., 2010), whic
h is a dwarf and present a rapid cycling
and a continuous production of fruits. Gene express
ion profile was studied on two pre - and
two post-véraison stages of berry development using
a whole genome microarray assay
(Nimblegen® vitis 12x). Equivalent developmental st
ages were sampled at day and night
time. A total number of 9273 probesets were develop
mentally modulated between all
stages day and night with a high number of genes be
ing modulated specifically at night
(1755). Eight very similar clusters of gene express
ion profiles could be obtained
independently for day and night development. Our st
udy allowed highlighting development
stage specifics mechanisms of diurnal modulation of
genes in the fruit that have been
overlooked in other transcriptomic studies on fruit
development so far. In the green berry
functional enrichment analysis indicated major chan
ges in cellular organization and
photosynthesis whereas in the ripe berry mainly sec
ondary metabolism was affected during
the night.
2017-03-21T10:03:12Z
2017-03-21T10:03:12Z
2013-09
póster de congreso
2nd Annual ConferenceFleshy Fruit Development & Ripening (2013)
http://hdl.handle.net/10261/147025
eng
Sí
closedAccess