2024-03-29T06:50:04Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1467922021-12-27T16:19:12Zcom_10261_86com_10261_1col_10261_339
2017-03-15T12:04:47Z
urn:hdl:10261/146792
Evidence of the presence of nucleic acids and β-glucan in the matrix of non-typeable Haemophilus influenzae in vitro biofilms
Domenech, Mirian
Pedrero-Vega, Elena
Prieto, Alicia
García, Ernesto
Ministerio de Economía y Competitividad (España)
Centro de Investigación Biomédica en Red Enfermedades Respiratorias (España)
Instituto de Salud Carlos III
13 p.-9 fig.
Non-typeable Haemophilus influenzae (NTHi) is a Gram-negative bacterium that frequently colonizes the human nasopharynx; it is a common cause of chronic and recurrent otitis media in children and of exacerbations of chronic obstructive pulmonary disease. To date, no exopolysaccharide clearly contributing to NTHi biofilms has been identified. Consequently, there is some debate as to whether NTHi forms biofilms during colonization and infection. The present work shows that NTHi can form
biofilms in vitro, producing an extracellular matrix composed of proteins, nucleic acids, and a β-glucan. Extracellular DNA, visualized by immunostaining and using fluorochromes, is an important component of this matrix and appears to be essential in biofilm maintenance. Extracellular RNA appears to be required only in the first steps of biofilm formation. Evidence of a matrix polysaccharide was obtained by staining with Calcofluor white M2R and by disaggregating biofilms with cellulase. Using strain
54997, residues of Glcp(1→4) in the NTHi biofilm were confirmed by gas-liquid chromatography-mass spectrometry. Evidence that N-acetyl-L-cysteine shows notable killing activity towards in vitro NTHi biofilm-forming bacteria is also provided.
2017-03-15T12:04:47Z
2017-03-15T12:04:47Z
2016-11-02
artículo
Scientific Reports 6:36424 (2016)
2045-2322
http://hdl.handle.net/10261/146792
10.1038/srep36424
2045-2322
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100004587
27805043
eng
Publisher's version
http://dx.doi.org/10.1038/srep36424
Sí
https://creativecommons.org/licenses/by/4.0/
openAccess
Nature Publishing Group