2024-03-29T05:39:24Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1350372023-02-01T07:46:15Zcom_10261_86com_10261_1col_10261_339
2016-07-26T09:36:18Z
urn:hdl:10261/135037
Genome sequence of the olive tree, Olea europaea
Cruz, Fernando
Cano Cabezas, Emilio
Galán, Beatriz
García, José Luis
Vargas, Pablo
Gabaldón, Toni
Banco Santander
Olive tree genome
Genomics
Assembly
Annotation
12 p.-6 fig.-5 tab. Fernando Cruz et al.
Background: The Mediterranean olive tree (Olea europaea subsp. europaea) was one of the first trees to be domesticated and is currently of major agricultural importance in the Mediterranean region as the source of olive oil. The molecular bases underlying the phenotypic differences among domesticated cultivars, or between domesticated olive trees and their wild relatives, remain poorly understood. Both wild and cultivated olive
trees have 46 chromosomes (2n).
Findings: A total of 543 Gb of raw DNA sequence from whole genome shotgun sequencing, and a fosmid library containing 155,000 clones from a 1,000+ year-old olive tree (cv. Farga) were generated by Illumina sequencing using different combinations of mate-pair and pair-end libraries. Assembly gave a final genome
with a scaffold N50 of 443 kb, and a total length of 1.31 Gb, which represents 95 % of the estimated genome length (1.38 Gb). In addition, the associated fungus Aureobasidium pullulans was partially sequenced. Genome annotation, assisted by RNA sequencing from leaf, root, and fruit tissues at various stages, resulted in 56,349
unique protein coding genes, suggesting recent genomic expansion. Genome completeness, as estimated using the CEGMA pipeline, reached 98.79 %.
Conclusions: The assembled draft genome of O. europaea will provide a valuable resource for the study of the evolution and domestication processes of this important tree, and allow determination of the genetic bases of key phenotypic traits. Moreover, it will enhance breeding programs and the formation of new varieties.
2016-07-26T09:36:18Z
2016-07-26T09:36:18Z
2016
artículo
GigaScience (2016) 5:29
2047-217X
http://hdl.handle.net/10261/135037
10.1186/s13742-016-0134-5
2047-217X
http://dx.doi.org/10.13039/100010784
27346392
eng
Publisher's version
http://dx.doi.org/ 10.1186/s13742-016-0134-5
Sí
http://creativecommons.org/licenses/by/4.0/
openAccess
BioMed Central