2024-03-29T10:03:09Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/131922021-03-18T16:13:19Zcom_10261_63com_10261_6col_10261_316
2009-05-25T12:02:42Z
urn:hdl:10261/13192
Aplicación de la técnica de tRFLP para evaluar las variaciones en las comunidades bacterianas del rumen asociadas a la inclusión de aceites de girasol y pescado en la dieta de ovejas
Belenguer, Álvaro
Toral, Pablo G.
Frutos, Pilar
Rabanal, Benjamín
Hervás, Gonzalo
Lipid suplementation
Molecular technique
Rumen bacteria
Ewe
3 págs, 1 fig.-- Trabajo presentado a las XIII Jornadas sobre Producción Animal (AIDA. Zaragoza 12-13 de mayo de 2009).
3 pages, 1 figure.
[EN] Four adult cannulated Merino ewes were fed a mixed diet (35:65
forage:concentrate) for 10 days. Afterwards they received the same diet supplemented with
sunflower (2%) and fish (1%) oils in order to study the effect of the lipid supplementation on
the rumen microbial communities. Rumen samples were taken after 0 (day 1), 3 (day 4) and
11 (day 12) days of the lipid supplementation. DNA was extracted and analysed by tRFLP
(qualitative molecular technique). An effect of the experimental treatment was observed,
being samples obtained on day 12 clustered separately from initial samples (day 1). On day
4, only two animals showed a similar microbial profile than on day 12, suggesting that the
time necessary for the rumen ecosystem to be adapted to the lipid supplementation may
depend on the individual microbial composition. Major fragments detected by tRFLP match
the abundant bacterial phyla Firmicutes and Bacteroidetes, and variations in other fragments
which may correspond to bacteria involved in rumen biohydrogenation (such as those
belonging to the Clostridial cluster IX or the family Lachnospiraceae) were detected. These
bacteria might be responsible for variations in the milk fatty acid profile in lactating ewes.
2009-05-25T12:02:42Z
2009-05-25T12:02:42Z
2009-05
comunicación de congreso
XIII Jornadas sobre Producción Animal AIDA. Tomo I: 754-756 (2009)
1130-6009
http://hdl.handle.net/10261/13192
spa
openAccess
Asociación Interprofesional para el Desarrollo Agrario