2024-03-28T23:16:50Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1290992020-01-13T11:52:27Zcom_10261_128com_10261_1col_10261_381
2016-02-15T14:23:02Z
urn:hdl:10261/129099
Dynamics of enhancer chromatin signatures mark the transition from pluripotency to cell specification during embryogenesis
Bogdanovic, Ozren
Fernández-Miñán, Ana
Tena, Juan J.
Calle-Mustienes, Elisa de la
Hidalgo, Carmen
Veenstra, Gert Jan C.
Gómez-Skarmeta, José Luis
Junta de Andalucía
This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date; after six months, it is available under a Creative Commons License.-- et al.
The generation of distinctive cell types that form different tissues and organs requires precise, temporal and spatial control of gene expression. This depends on specific cis-regulatory elements distributed in the noncoding DNA surrounding their target genes. Studies performed on mammalian embryonic stem cells and Drosophila embryos suggest that active enhancers form part of a defined chromatin landscape marked by histone H3 lysine 4 mono-methylation (H3K4me1) and histone H3 lysine 27 acetylation (H3K27ac). Nevertheless, little is known about the dynamics and the potential roles of these marks during vertebrate embryogenesis. Here, we provide genomic maps of H3K4me1/me3 and H3K27ac at four developmental time-points of zebrafish embryogenesis and analyze embryonic enhancer activity. We find that (1) changes in H3K27ac enrichment at enhancers accompany the shift from pluripotency to tissue-specific gene expression, (2) in early embryos, the peaks of H3K27ac enrichment are bound by pluripotent factors such as Nanog, and (3) the degree of evolutionary conservation is higher for enhancers that become marked by H3K27ac at the end of gastrulation, suggesting their implication in the establishment of the most conserved (phylotypic) transcriptome that is known to occur later at the pharyngula stage.
2016-02-15T14:23:02Z
2016-02-15T14:23:02Z
2012
artículo
Genome Research 22(10): 2043-2053 (2012)
1088-9051
http://hdl.handle.net/10261/129099
10.1101/gr.134833.111
1549-5469
http://dx.doi.org/10.13039/501100011011
22593555
eng
http://dx.doi.org/10.1101/gr.134833.111
Sí
http://creativecommons.org/licenses/by-nc/3.0/
openAccess
Cold Spring Harbor Laboratory Press