2024-03-19T08:17:22Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1290792021-12-28T16:08:13Zcom_10261_128com_10261_1col_10261_381
2016-02-15T10:36:57Z
urn:hdl:10261/129079
Comparative genomics of the Hedgehog loci in chordates and the origins of Shh regulatory novelties
Irimia, Manuel
Royo, José Luis
Burguera, Demian
Maeso, Ignacio
Gómez-Skarmeta, José Luis
Garcia-Fernàndez, Jordi
Consejo Superior de Investigaciones Científicas (España)
European Commission
Ministerio de Economía y Competitividad (España)
Ministerio de Ciencia e Innovación (España)
Institución Catalana de Investigación y Estudios Avanzados
Junta de Andalucía
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareALike 3.0 Unported License.
The origin and evolution of the complex regulatory landscapes of some vertebrate developmental genes, often spanning hundreds of Kbp and including neighboring genes, remain poorly understood. The Sonic Hedgehog (Shh) genomic regulatory block (GRB) is one of the best functionally characterized examples, with several discrete enhancers reported within its introns, vast upstream gene-free region and neighboring genes (Lmbr1 and Rnf32). To investigate the origin and evolution of this GRB, we sequenced and characterized the Hedgehog (Hh) loci from three invertebrate chordate amphioxus species, which share several early expression domains with Shh. Using phylogenetic footprinting within and between chordate lineages, and reporter assays in zebrafish probing >30 Kbp of amphioxus Hh, we report large sequence and functional divergence between both groups. In addition, we show that the linkage of Shh to Lmbr1 and Rnf32, necessary for the unique gnatostomate-specific Shh limb expression, is a vertebrate novelty occurred between the two whole-genome duplications.
2016-02-15T10:36:57Z
2016-02-15T10:36:57Z
2012
2016-02-15T10:36:59Z
artículo
Scientific Reports 2: 433 (2012)
http://hdl.handle.net/10261/129079
10.1038/srep00433
http://dx.doi.org/10.13039/501100003339
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100004837
http://dx.doi.org/10.13039/501100003741
http://dx.doi.org/10.13039/501100011011
22666536
eng
Publisher's version
http://dx.doi.org/10.1038/srep00433
Sí
http://creativecommons.org/licenses/by-nc-sa/3.0/
openAccess
Nature Publishing Group