2024-03-29T13:38:55Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1253762021-12-28T16:00:16Zcom_10261_5063com_10261_5col_10261_5066
2015-11-20T08:23:43Z
urn:hdl:10261/125376
Variable-Temperature Size Exclusion Chromatography for the Study of the Structural Changes in G-Quadruplex
Benabou, Sanae
Gargallo, Raimundo
Eritja Casadellà, Ramón
G-quadruplex
Chromatography
The conformational equilibria of a guanine-rich sequence found at the promoter region of the human c-kit oncogene are studied by means of circular dichroism spectroscopy (CD) and variable-temperature size exclusion chromatography (SEC). It is shown that the wild sequence ckit21 exists as a mixture of monomeric and multimeric G-quadruplexes. Appropriate mutation of several bases in the wild sequence produces the shift from parallel to antiparallel G-quadruplex, as well as the disappearance of multimeric species. The shift from the antiparallel to the parallel conformation induced by temperature is reflected in both CD and SEC profiles.
2015-11-20T08:23:43Z
2015-11-20T08:23:43Z
2013
artículo
ISRN Biochemistry
http://hdl.handle.net/10261/125376
10.1155/2013/631875
25937962
eng
Publisher's version
http://dx.doi.org/10.1155/2013/631875
Sí
http://www.sherpa.ac.uk/romeo/issn/2090-7729/
openAccess
Hindawi Publishing Corporation