2024-03-29T08:40:53Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1243752017-11-07T11:50:32Zcom_10261_22com_10261_1col_10261_905
2015-11-03T12:30:04Z
urn:hdl:10261/124375
Differential plant proteome analysis by isobaric tags for relative and absolute quantitation (iTRAQ)
Martínez-Esteso, María J.
Casado-Vela, Juan
Sellés, Susana
Pedreño, María A.
Bru-Martínez, Roque
Ministerio de Ciencia e Innovación (España)
European Commission
Fundación Cajamurcia
Universidad de Alicante
Instituto de Salud Carlos III
Red Temática de Investigación Cooperativa en Cáncer (España)
Protein relative quantitation is one of the main targets in many proteomic experiments. Among the range of techniques available for both top-down and bottom-up approaches, isobaric tags for relative and absolute quantitation (iTRAQ) have gained positions within the top-rank techniques used for this purpose in the recent years. Briefly, each iTRAQ reagent consists of three different components: a reporter group (with a variable mass in the range of 114-117 amu), a balance group, and an amino-reactive group. The isobaric nature of iTRAQ-labeled peptides adds a signal to every peptide in the sample which is detectable in both MS and MS/MS spectra, thus enhancing the sensitivity of detection. During MS/MS, the reporter groups are released as singly charged ions with m/z ratios ranking from 114 to 117 amu, visible in the low mass region of MS/MS spectra. The iTRAQ technology can be used to analyze up to four different samples using the 4-plex kit (reporter groups 114-115 amu) or can be scaled up to eight different samples using the 8-plex kit (reporter groups 113-121 amu). In this chapter, we focus on the experimental procedures typically using 4-plex labeling, including tips leading to successful application of iTRAQ technology for the analysis of plant protein mixtures.
2015-11-03T12:30:04Z
2015-11-03T12:30:04Z
2014
2015-11-03T12:30:05Z
capítulo de libro
Plant Proteomics (Cap.12): 155-169 (2014)
http://hdl.handle.net/10261/124375
10.1007/978-1-62703-631-3_12
http://dx.doi.org/10.13039/501100004837
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/100009092
http://dx.doi.org/10.13039/501100004587
http://dx.doi.org/10.13039/501100008540
eng
Methods in Molecular Biology
1072
Sí
closedAccess
Springer