2024-03-29T12:01:52Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1231172021-12-27T16:21:38Zcom_10261_64com_10261_1col_10261_317
2015-10-07T11:26:59Z
urn:hdl:10261/123117
In vitro characterization of a miR-122-sensitive double-helical switch element in the 5′ region of hepatitis C virus RNA
Díaz-Toledano, Rosa
Ariza-Mateos, Ascensión
Birk, A.
Martínez-García, Belén
Gómez, Jordi
Ministerio de Ciencia e Innovación (España)
Junta de Andalucía
Centro de Investigación Biomédica en Red Enfermedades Hepáticas y Digestivas (España)
Fundación para la Investigación y la Prevención del Sida en España
National Institutes of Health (US)
Supplementari Data http://nar.oxfordjournals.org/content/suppl/2009/10/12/gkp553.DC1
It has been proposed that the hepatitis C virus (HCV) internal ribosome entry site (IRES) resides within a locked conformation, owing to annealing of its immediate flanking sequences. In this study, structure probing using Escherichia coli dsRNA-specific RNase III and other classical tools showed that this region switches to an open conformation triggered by the liver-specific microRNA, miR-122. This structural transition, observed in vitro, may be the mechanistic basis for the involvement of downstream IRES structural domain VI in translation, as well as providing a role of liver-specific miR-122 in HCV infection. In addition, the induced RNA switching at the 5′ untranslated region could ultimately represent a new mechanism of action of micro-RNAs.
2015-10-07T11:26:59Z
2015-10-07T11:26:59Z
2009-07
artículo
Nucleic Acids Research
0305-1048
http://hdl.handle.net/10261/123117
10.1093/nar/gkp553
1362-4962
http://dx.doi.org/10.13039/501100004837
http://dx.doi.org/10.13039/501100007671
http://dx.doi.org/10.13039/100000002
http://dx.doi.org/10.13039/501100011011
19578061
eng
Publisher's version
http://nar.oxfordjournals.org/content/early/2009/07/03/nar.gkp553.full
Sí
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
openAccess
Oxford University Press