2024-03-29T00:16:33Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1153632016-11-18T10:12:08Zcom_10261_75com_10261_6col_10261_328
urn:hdl:10261/115363
Pigment stoichiometry of a newly isolated D1-D2-Cyt b559 complex from the higher plant Beta vulgaris L.
Montoya Blanco, Guillermo
Yruela Guerrero, Inmaculada
Picorel Castaño, Rafael
Beta vulgaris
Stoichiometry
Pigment
Reaction center
Photosystem II
Two D1-D2-Cyt b559 complexes with different pigment stoichiometry were isolated from the higher plant B. vulgaris. The procedures for isolating both complexes only differed in the washing time of the DEAE column with 50 mM Tris-HCl, pH 7.2, 0.05% Triton X-100 and 30 mM NaCl. When the column was washed until the eluate had an absorbance of 0.01 at 670 nm, the isolated D1-D2-Cyt b559 complex presented a pigment stoichiometry of 6 chlorophyll a, 2 β-carotene, and 1 cytochrome b559 per 2 pheophytin a. In contrast, when the column was exhaustively washed until the eluate reached an absorbance of 0.005 at 670 nm, the complex had a stoichiometry of 4 chlorophyll a, 1 β-carotene, and 1 cytochrome b559 per 2 pheophytin a. We think that the former stoichiometry corresponds to that of the native Da-D2-Cyt b559 complex. Moreover, both preparations showed 2 mol of pheophytin a per 1 mol of reaction center protein.
1991
2015-05-19T07:39:52Z
artículo
FEBS Letters 283: 255- 258 (1991)
http://hdl.handle.net/10261/115363
10.1016/0014-5793(91)80601-X
eng
http://dx.doi.org/10.1016/0014-5793(91)80601-X
closedAccess
Elsevier