2024-03-28T18:18:59Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1118632021-12-28T16:35:35Zcom_10261_80com_10261_1col_10261_333
2015-03-05T10:38:09Z
urn:hdl:10261/111863
Alr0397 Is an Outer Membrane Transporter for the Siderophore Schizokinen in Anabaena sp. Strain PCC 7120
Nicolaisen, K.
Moslavac, S.
Samborski, A.
Valdebenito, M.
Hantke, K.
Maldener, Iris
Muro-Pastor, Alicia M.
Flores, Enrique
Schleiff, E.
Iron uptake in proteobacteria by TonB-dependent outer membrane transporters represents a well-explored
subject. In contrast, the same process has been scarcely investigated in cyanobacteria. The heterocyst-forming
cyanobacterium Anabaena sp. strain PCC 7120 is known to secrete the siderophore schizokinen, but its
transport system has remained unidentified. Inspection of the genome of strain PCC 7120 shows that only one
gene encoding a putative TonB-dependent iron transporter, namely alr0397, is positioned close to genes
encoding enzymes involved in the biosynthesis of a hydroxamate siderophore. The expression of alr0397, which
encodes an outer membrane protein, was elevated under iron-limited conditions. Inactivation of this gene
caused a moderate phenotype of iron starvation in the mutant cells. The characterization of the mutant strain
showed that Alr0397 is a TonB-dependent schizokinen transporter (SchT) of the outer membrane and that
alr0397 expression and schizokinen production are regulated by the iron homeostasis of the cell.
2015-03-05T10:38:09Z
2015-03-05T10:38:09Z
2008
artículo
Journal of Bacteriology, 190(22): 7500-7507 (2008)
0021-9193
http://hdl.handle.net/10261/111863
10.1128/JB.01062-08
18805987
eng
Publisher's version
http://dx.doi.org/10.1128/JB.01062-08
Sí
openAccess
American Society for Microbiology