2024-03-29T10:49:31Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1033802021-12-28T16:16:06Zcom_10261_86com_10261_1col_10261_339
2014-10-15T11:06:24Z
urn:hdl:10261/103380
Interactions between the RepB initiator protein of plasmid pMV158 and two distant DNA regions within the origin of replication
Ruiz-Masó, José A.
Lurz, Rudi
Espinosa, Manuel
Solar, Gloria del
15 p.-7 fig.-1 tab.
Plasmids replicating by the rolling circle mode usually possess a single site for binding of the initiator protein at the origin of replication. The origin of pMV158 is different in that it possesses two distant binding regions for the initiator RepB. One region was located close to the site where RepB introduces the replication-initiating nick, within the nic locus; the other, the bind locus, is 84 bp downstream from the nick site. Binding of RepB to the bind locus was of higher affinity and
stability than to the nic locus. Contacts of RepB with the bind and nic loci were determined through high-resolution footprinting. Upon binding of RepB, the DNA of the bind locus follows a winding path in its contact with the protein, resulting in local distortion and bending of the double-helix. On supercoiled DNA, simultaneous interaction of RepB with both loci favoured extrusion of the hairpin structure harbouring the nick site while causing a strong DNA distortion around the bind locus. This suggests interplay between the two RepB binding sites, which could facilitate loading
of the initiator protein to the nic locus and
the acquisition of the appropriate configuration of the supercoiled DNA substrate.
2014-10-15T11:06:24Z
2014-10-15T11:06:24Z
2007
artículo
Nucleic Acids Research 2007, 35 (4) 1230–1244
0305-1048
http://hdl.handle.net/10261/103380
10.1093/nar/gkl1099
1362-4962)
17267412
eng
Publisher's version
http://dx.doi.org/10.1093/nar/gkl1099
Sí
http://creativecommons.org/licenses/by-nc-nd/3.0
openAccess
Oxford University Press