2024-03-29T00:34:12Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1021832022-11-16T08:02:38Zcom_10261_11com_10261_6col_10261_264
2014-09-17T10:35:54Z
urn:hdl:10261/102183
Divergent selection for ester-linked diferulates in maize pith stalk tissues. Effects on cell wall composition and degradability
Barros Ríos, Jaime Antonio
Malvar Pintos, Rosa Ana
Jung, Hans-Joachim G.
Bunzel, Mirko
Santiago Carabelos, Rogelio
Zea mays
Divergent mass selection
Hydroxycinnamic acids
Ferulate cross-links
Lignin
In vitro biodegradability
Cross-linking of grass cell wall components through diferulates (DFAs) has a marked impact on cell wall properties. However, results of genetic selection for DFA concentration have not been reported for any grass species. We report here the results of direct selection for ester-linked DFA concentration in maize stalk pith tissues and the associated changes in cell wall composition and biodegradability. After two cycles of divergent selection, maize populations selected for higher total DFA (DFAT) content (CHs) had 16% higher DFAT concentrations than populations selected for lower DFAT content (CLs). These significant DFA concentration gains suggest that DFA deposition in maize pith parenchyma cell walls is a highly heritable trait that is genetically regulated and can be modified trough conventional breeding. Maize populations selected for higher DFAT had 13% less glucose and 10% lower total cell wall concentration than CLs, suggesting that increased cross-linking of feruloylated arabinoxylans results in repacking of the matrix and possibly in thinner and firmer cell walls. Divergent selection affected esterified DFAT and monomeric ferulate ether cross link concentrations differently, supporting the hypothesis that the biosynthesis of these cell wall components are separately regulated. As expected, a more higher DFA ester cross-coupled arabinoxylan network had an effect on rumen cell wall degradability (CLs showed 12% higher 24-h total polysaccharide degradability than CHs). Interestingly, 8–8-coupled DFAs, previously associated with cell wall strength, were the best predictors of pith cell wall degradability (negative impact). Thus, further research on the involvement of these specific DFA regioisomers in limiting cell wall biodegradability is encouraged.
2014-09-17T10:35:54Z
2014-09-17T10:35:54Z
2012-11
artículo
Phytochemistry 83: 43–50 (2012)
0031-9422
http://hdl.handle.net/10261/102183
10.1016/j.phytochem.2012.07.026
1873-3700
eng
http://dx.doi.org/10.1016/j.phytochem.2012.07.026
Sí
closedAccess
Elsevier