2024-03-29T05:42:06Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/799952023-02-06T12:54:10Zcom_10261_22com_10261_1col_10261_275
http://hdl.handle.net/10261/79995
10.1016/S0014-5793(02)02760-6
84202
TGF-β1 and Smad4 overexpression induce a less invasive phenotype in highly invasive spindle carcinoma cells
Elsevier
2002
artículo
Santibáñez, Juan F.
Quintanilla, Miguel
Martínez, Jorge
2002
We have examined the effect of transforming growth factor β1 (TGF-β1) and overexpression of the Smad4 gene on the phenotype of Car C, a ras mutated highly malignant spindle carcinoma cell line. TGF-β1-treated Car C cells overexpressing Smad4 spread with a flattened morphology with membrane ruffles abundant in vinculin and show a reduction in their invasive abilities. TGF-β1 treatment and overexpression of Smad4 also enhanced the production of PAI-1 measured by the activation of the p3TP-lux reporter gene containing a PAI-1-related promoter. This activation was abolished with a dominant-negative Smad4 construct. These results lead us to conclude that both TGF-β1 and Smad4 overexpression reduce the invasive potential of Car C cells, probably via the Smad pathway.
FEBS Letters 520(1-3)
2002
171
176