2024-03-28T18:58:37Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/743382017-02-16T11:27:57Zcom_10261_75com_10261_6com_10261_10735com_10261_2col_10261_328col_10261_10736
http://hdl.handle.net/10261/74338
10.1002/jpln.201100393
77999
Nitrogen metabolism is related to improved water-use efficiency of nodulated alfalfa grown with sewage sludge under drought
John Wiley & Sons
2013
artículo
Fiasconaro, M. Laura
Sánchez-Díaz, Manuel
Antolín Bellver, M. Carmen
Medicago sativa L.
Nitrate assimilation
N2 fixation
Photosynthesis
Water deficit
2013-01
Leguminous plants grown in sewage sludge-amended soils can acquire nitrogen by assimilation of nitrate and ammonium from the soil solution or from atmospheric-dinitrogen (N2) fixation through association with N2-fixing bacteria. We proposed that operation of both metabolic processes could contribute to alleviate the impact of drought in sludge-treated plants. A greenhouse experiment was conducted to evaluate the involvement of nodule metabolism in the use efficiency of water and N in sludge-treated plants. Treatments comprised (1) plants inoculated with rhizobia and amended with sewage sludge; (2) plants inoculated with rhizobia without any amendment; and (3) noninoculated plants supplied with ammonium nitrate, each under well-watered and drought conditions. Under drought, sludge-treated plants had increased plant growth and higher photosynthetic and water-use efficiencies than untreated plants. Drought stimulated nitrate reductase and GS/GOGAT activities but did not affect the activities of phosphoenolpyruvate carboxylase and malate dehydrogenase or the leghemoglobin concentration. The results suggest that under drought conditions, both N2 fixation and nitrate assimilation in nodules of sludge-treated plants contributed to improve plant N supply and to increase the drought tolerance of alfalfa. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Asociación de Amigos de la Universidad de Navarra
Navarra de Infraestructuras Locales
Journal of Plant Nutrition and Soil Science
2013
176
110
117