2024-03-29T06:41:52Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/2123672021-04-13T15:13:18Zcom_10261_63com_10261_6col_10261_316
http://hdl.handle.net/10261/212367
10.1016/j.rvsc.2019.01.004
393087
In vitro anthelmintic activity and safety of different plant species against the ovine gastrointestinal nematode Teladorsagia circumcincta
Elsevier
2019
artículo
Esteban Ballesteros, M.
Sanchis, J.
Gutiérrez-Corbo, C.
Balaña-Fouce, Rafael
Rojo Vázquez, Francisco Antonio
González Lanza, Camino
rp08087
Martínez Valladares, María
rp14113
Plant extract
Egg hatch assay
Anthelmintic activity
Cytotoxicity
Teladorsagia circumcincta
2019
6 páginas, 4 tablas.
The present study was conducted to evaluate the in vitro anthelmintic activity and safety of methanolic and aqueous extracts of 9 plants against the ovine GIN Teladorsagia circumcincta. Initially the ovicidal efficacy of all extracts was tested at 50 mg/ml and at 1%, this last concentration for the methanolic extract of Elettaria cardamomum, by the Egg Hatch Assay. In those extracts with efficacy higher than 95%, the effective concentration required to inhibit 50% of egg hatching (EC) and their cytotoxicity, as the lethal dose 50 (LC), was also measured. The aqueous extracts of Aesculus hippocastanum, Isatis tinctoria, Chelidonium majus, E. cardamomum and Sisymbrium irio, with EC values ranging 1.30–2.88 mg/ml, and the aqueous extract of Jasminum polyanthum with a value of 6.41 mg/ml, showed the highest activities. The aqueous extract of J. polyanthum was the safest extract, followed by methanolic extract of E. cardamomum and aqueous extract of S. irio, all of them with a Selective Index higher than 1. According to our results, there is no correlation between the amount of total phenols or total tannins with the anthelmintic activity of the plants tested. Although results need to be interpreted with caution, as in vitro activity may not automatically translate into in vivo efficacy, those extracts with SI equal or higher than 1 and EC equal or lower than 6 mg/ml, could be taken into account for being used subsequently as feed or food additives in infected sheep.
CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)
Ministerio de Economía y Competitividad (España)
European Commission
Research in Veterinary Science
2019
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153
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