2024-03-29T04:48:58Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1929462021-12-27T15:56:53Zcom_10261_94com_10261_8col_10261_347
http://hdl.handle.net/10261/192946
10.1186/s13071-019-3746-7
373210
Disruption of gut integrity and permeability contributes to enteritis in a fish-parasite model: a story told from serum metabolomics
BioMed Central
2019
artículo
Sitjà-Bobadilla, Ariadna
rp04142
Gil-Solsona, Rubén
Estensoro, Itziar
Piazzon de Haro, María Carla
rp09261
Martos-Sitcha, Juan Antonio
Picard-Sánchez, Amparo
Fuentes, Juan
Sancho, Juan Vicente
Calduch-Giner, Josep A.
Hernández, F.
Pérez-Sánchez, Jaume
Myxozoa
Enteromyxum leei
Gilthead sea bream
Teleostei
Aquaculture
Pathophysiology
Tight junctions
Gut barrier
Electrophysiology
Metabolomics
2019-10-16
© The Author(s).
[Background]:In the animal production sector, enteritis is responsible for serious economic losses, and intestinal parasitism is a major stress factor leading to malnutrition and lowered performance and animal production efficiency. The effect of enteric parasites on the gut function of teleost fish, which represent the most ancient bony vertebrates, is far from being understood. The intestinal myxozoan parasite Enteromyxum leei dwells between gut epithelial cells and causes severe enteritis in gilthead sea bream (Sparus aurata), anorexia, cachexia, growth impairment, reduced marketability and increased mortality.
[Methods]: This study aimed to outline the gut failure in this fish-parasite model using a multifaceted approach and to find and validate non-lethal serum markers of gut barrier dysfunction. Intestinal integrity was studied in parasitized and non-parasitized fish by immunohistochemistry with specific markers for cellular adhesion (E-cadherin) and tight junctions (Tjp1 and Cldn3) and by functional studies of permeability (oral administration of FITC-dextran) and electrophysiology (Ussing chambers). Serum samples from parasitized and non-parasitized fish were analyzed using non-targeted metabolomics and some significantly altered metabolites were selected to be validated using commercial kits.
[Results]: The immunodetection of Tjp1 and Cldn3 was significantly lower in the intestine of parasitized fish, while no strong differences were found in E-cadherin. Parasitized fish showed a significant increase in paracellular uptake measured by FITC-dextran detection in serum. Electrophysiology showed a decrease in transepithelial resistance in infected animals, which showed a diarrheic profile. Serum metabolomics revealed 3702 ions, from which the differential expression of 20 identified compounds significantly separated control from infected groups in multivariate analyses. Of these compounds, serum inosine (decreased) and creatine (increased) were identified as relevant and validated with commercial kits.
[Conclusions]: The results demonstrate the disruption of tight junctions and the loss of gut barrier function, a metabolomic profile of absorption dysfunction and anorexia, which further outline the pathophysiological effects of E. leei.
Ministerio de Economía y Competitividad (España)
Generalitat Valenciana
Fundação para a Ciência e a Tecnologia (Portugal)
European Commission
Parasites & Vectors
2019
12
486