2024-03-29T13:32:06Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1904402019-09-27T10:37:43Zcom_10261_101com_10261_5col_10261_354
http://hdl.handle.net/10261/190440
10.1007/s00248-015-0606-7
370639
Conversion of Uric Acid into Ammonium in Oil-Degrading Marine Microbial Communities: a Possible Role of Halomonads
Springer
2015
artículo
Gertler, Christoph
Bargiela, Rafael
Mapelli, Francesca
Han, Xifang
Chen, Jianwei
Hai, Tran
Amer, Ranya A.
Mahjoubi, Mouna
Malkawi, Hanan I.
Magagnini, Mirko
Cherifc, Ameur
Abdel-Fattah, Yasser R
Kalogerakis, Nicolas
Daffonchio, Daniele
Ferrer, Manuel
Golyshin, Peter N.
Bioremediation
Alcanivorax
Crude oil degradation
2015-10
Uric acid is a promising hydrophobic nitrogen source for biostimulation of microbial activities in oil-impacted marine environments. This study investigated metabolic processes and microbial community changes in a series of microcosms using sediment from the Mediterranean and the Red Sea amended with ammonium and uric acid. Respiration, emulsification, ammonium and protein concentration measurements suggested a rapid production of ammonium from uric acid accompanied by the development of microbial communities containing hydrocarbonoclastic bacteria after 3 weeks of incubation. About 80 % of uric acid was converted to ammonium within the first few days of the experiment. Microbial population dynamics were investigated by Ribosomal Intergenic Spacer Analysis and Illumina sequencing as well as by culture-based techniques. Resulting data indicated that strains related to Halomonas spp. converted uric acid into ammonium, which stimulated growth of microbial consortia dominated by Alcanivorax spp. and Pseudomonas spp. Several strains of Halomonas spp. were isolated on uric acid as the sole carbon source showed location specificity. These results point towards a possible role of halomonads in the conversion of uric acid to ammonium utilized by hydrocarbonoclastic bacteria.
European Commission
Ministerio de Economía y Competitividad (España)
Regione Lombardia
King Abdullah University of Science and Technology
Università degli Studi di Milano
Microbial Ecology
2015
70
724
740