2024-03-28T20:44:20Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1809492021-04-30T12:46:54Zcom_10261_47com_10261_8col_10261_300
http://hdl.handle.net/10261/180949
10.1016/j.jprot.2019.04.006
360894
Proteome Profiling of L3 and L4 Anisakis simplex Development Stages by TMT-based Quantitative Proteomics
Elsevier
2019
artículo
Stryiński, Robert
Mateos, Jesús
Pascual, Santiago
González, Ángel F.
Gallardo, José Manuel
Lopieńska-Biernat, Elzbieta
Medina, Isabel
rp14008
Carrera, Mónica
rp13661
Stryiński, Robert [0000-0001-5518-8854]
Mateos, Jesús [0000-0002-1782-6779]
Carrera, Mónica [0000-0003-2973-449X]
Anisakis simplex
Anisakiosis
Proteome
Quantitative proteomics
Tandem mass tag (TMT)
L3/L4 development stage
2019
11 pages, 8 figures, 2 tables
Anisakis simplex is a parasitic nematode that can cause anisakiosis and/or allergic reactions in humans. The presence of invasive third-stage larvae (L3) in many different consumed fish species and the fourth-stage larvae (L4) in marine mammals, where L3 can accidentally affect to humans and develop as far as stage L4. World Health Organization and food safety authorities aim to control and prevent this emerging health problem. In the present work, using Tandem Mass Tag (TMT)-based quantitative proteomics we analyzed for the first time the global proteome of two A. simplex development stages, L3 and L4. The strategy was divided into four steps: (a) protein extraction of L3 and L4 development stages, (b) high intensity focused ultrasound (HIFU)-assisted trypsin digestion, (c) TMT-isobaric mass tag labeling following by high-pH reversed-phase fractionation, and (d) LC-MS/MS analysis in a LTQ-Orbitrap Elite mass spectrometer. A total of 2443 different proteins of A. simplex were identified. Analysis of the modulated proteins provided the specific proteomic signature of L3 (i.e. pseudocoelomic globin, endochitinase 1, paramyosin) and L4 (i.e. neprilysin-2, glutamate dehydrogenase, aminopeptidase N). To our knowledge, this is the most comprehensive dataset of proteins of A. simplex for two development stages (L3 and L4) identified to date
Journal of Proteomics
2019
201
1
11