2024-03-29T14:06:33Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1298642022-06-01T10:58:24Zcom_10261_5063com_10261_5col_10261_5066
http://hdl.handle.net/10261/129864
10.1039/c4ob02087a
308949
Niosomes based on synthetic cationic lipids for gene delivery: The influence of polar head-groups on the transfection efficiency in HEK-293, ARPE-19 and MSC-D1 cells
Royal Society of Chemistry (UK)
2015
artículo
Ojeda, Edilberto
Puras, Gustavo
Agirre, Mireia
Zárate, Jon
Grijalvo, Santiago
Pons Pons, Ramon
Eritja Casadellà, Ramón
rp13637
Martínez Navarrete, Gema C.
Soto-Sánchez, Cristina
Fernández, E.
Pedraz, Jose Luis
Cells
Genes
Molecular biology
genetic transfection
Lipids
2015-04-14
We designed niosomes based on three lipids that differed only in the polar-head group to analyze their influence on the transfection efficiency. These lipids were characterized by small-angle X-ray scattering before being incorporated into the niosomes which were characterized in terms of pKa, size, zeta potential, morphology and physical stability. Nioplexes were obtained upon the addition of a plasmid. Different ratios (w/w) were selected to analyze the influence of this parameter on size, charge and the ability to condense, release and protect the DNA. In vitro transfection experiments were performed in HEK-293, ARPE-19 and MSC-D1 cells. Our results show that the chemical composition of the cationic head-group clearly affects the physicochemical parameters of the niosomes and especially the transfection efficiency. Only niosomes based on cationic lipids with a dimethyl amino head group (lipid 3) showed a transfection capacity when compared with their counterparts amino (lipid 1) and tripeptide head-groups (lipid 2). Regarding cell viability, we clearly observed that nioplexes based on the cationic lipid 3 had a more deleterious effect than their counterparts, especially in ARPE-19 cells at 20/1 and 30/1 ratios. Similar studies could be extended to other series of cationic lipids in order to progress in the research on safe and efficient non-viral vectors for gene delivery purposes.
Organic and Biomolecular Chemistry