2024-03-19T06:58:45Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1239242018-07-20T09:30:47Zcom_10261_133com_10261_1com_10261_22col_10261_386col_10261_275
http://hdl.handle.net/10261/123924
10.1016/j.celrep.2013.09.030
302874
eEF1A mediates the nuclear export of SNAG-containing proteins via the exportin5-aminoacyl-tRNA complex
Elsevier
2013
artículo
Mingot, José Manuel
Vega, Sonia
Cano, Amparo
Portillo, Francisco
Nieto, M. Ángela
rp07301
2013
This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works
License.
Exportin5 mediates the nuclear export of double-stranded RNAs, including pre-microRNAs, adenoviral RNAs, and tRNAs. When tRNAs are aminoacylated, the Exportin5-aminoacyl (aa)-tRNA complex recruits and coexports the translation elongation factor eEF1A. Here, we show that eEF1A binds to Snail transcription factors when bound to their main target, the E-cadherin promoter, facilitating their export to the cytoplasm in association with the aa-tRNA-Exportin5 complex. Snail binds to eEF1A through the SNAG domain, a protein nuclear export signal present in several transcription factor families, and this binding is regulated by phosphorylation. Thus, we describe a nuclear role for eEF1A and provide a mechanism for protein nuclear export that attenuates the activity of SNAG-containing transcription factors. © 2013 The Authors.
Generalitat Valenciana
Ministerio de Ciencia e Innovación (España)
Cell Reports
2013
5
727
737