2024-03-29T07:46:05Zhttp://digital.csic.es/dspace-oai/requestoai:digital.csic.es:10261/1084962018-11-28T11:55:08Zcom_10261_70com_10261_2col_10261_323
http://hdl.handle.net/10261/108496
10.1016/j.tca.2014.08.015
255559
Glass transition and heat capacity behaviors of plant vitrification solutions
Elsevier
2014
artículo
Schneider Teixeira, Aline
Faltus, Miloš
González-Benito, M. Elena
Molina García, Antonio D.
rp09341
Zámecníkc, Jiri
Plant vitrification solution
TG
ΔCp
Cryopreservation
DSC
2014
Differential scanning calorimetry (DSC) was employed to investigate the vitrification and annealing behaviors of the most commonly used plant vitrification solutions (PVS). These solutions are employed to protect plant tissues toward ice formation and freeze injury, and help to the vitrification of these tissues, by globally reducing the intracellular fluids mobility. Glass transition temperatures (TG) and heat capacity increments (ΔCp) were determined for five solutions PVS1, PVS2, PVS2 mod, PVS3 and PVS3 mod, with different composition, and a range of cooling and warming rates was employed. Glass transitions showed clear and consistent temperature differences within vitrification solutions, which could be related to composition and water content. Roughly, two sets of TG values were obtained, those for PVS1 and PVS2, at −112 °C and −114 °C, respectively, and those for PVS3, at −90 °C. The observed TG and ΔCp, unexpectedly, did not significantly change within a wide range of cooling rates (from 5 °C min−1 to liquid nitrogen quenching) and warming rates (from 5 to 20 °C). Garlic shoot tips cryopreserved after the droplet method produced a similar result to that of the vitrification solutions employed. After quench cooling to temperatures below TG, repeated excursions to higher temperatures were made and the cooling and warming TG were recorded. These treatments had little or no effect over the PVS solutions TG, which remained practically constant. A direct practical consequence is that the plant vitrification solutions glass transition temperature does not significantly change with cryopreservation methods based on either direct plunging of samples into liquid nitrogen or employing closed cryovials.
Thermochimica Acta
2014
593
43
49