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Firma en Digital.CSIC (*)
Mellado, Mario
Centro o Instituto
CSIC - Centro Nacional de Biotecnología (CNB)
Inmunología y Oncología
The chemokines are a family of chemoattractant mediators with strong pharmacological interest, they bind to GPCR receptors and direct cell movement. They are essential for immune system homeostasis and key proteins in multiple inflammatory and autoimmune diseases such as rheumatoid arthritis, multiple sclerosis, ulcerative colitis, Crohn’s disease, etc. They have also been linked to HIV-1 infection, tumor metastasis and transplant rejection. After an initial stage studying the signaling cascades triggered by chemokines, he described, for the first time, that these inflammatory mediators activate the JAK/STAT pathway, a signaling pathway classically associated to the cytokine receptors. He also was pioneer describing that chemokine receptors form homo- and heterodimers at the cell surface. At that time, he published more than 50 manuscripts, signed 4 international patents and supervised 4 doctoral theses. In 2005, he obtained a permanent scientific position in the Spanish Research Council (CSIC) and stabilized his position as a group leader in the CNB. He consolidated his research on chemokines and incorporated new innovative imaging technologies, such as those based in resonant energy transfer (FRET and BRET). Using these techniques, he confirmed the existence of chemokine receptor complexes even in the absence of ligands and demonstrated that homo- and heterodimers were dynamic conformations regulated by receptor expression and ligand binding. At this stage, he supervised 8 doctoral theses and became interested in translational aspects of his research. He participated in 2 european consortia supported by the UE FP7 programme that facilitated an international development of his research. He also participated in 2 national consortia on rheumatic diseases, bringing his research closer to clinical and translational medicine. His commitment to incorporate new technologies in his research, led him to start working with TIRF techniques (total internal reflection microscopy) and super-resolution microscopy (STED). Now he uses these methodologies to analyze the spatial distribution and dynamics of the chemokine receptors in the cell membrane, as well as to identify those factors involved in their regulation. He is also interested in the dynamics of the signaling molecules associated to these receptors, as well as in the identification of potential targets and tools with therapeutic value to intervene in chemokine function. Currently he is also director of the CNB/CSIC.
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Results 1-20 of 31 (Search time: 0.05 seconds).

RightsPreviewIssue DateTitleAuthor(s)Type
1openAccessclinical_VIP_axis_inflammatory_autoimmune_diseases.pdf.jpg20-Dec-2019A clinical approach for the use of VIP axis in inflammatory and autoimmune diseasesMartínez, Carmen; Juarranz, Yasmina; Gutiérrez-Cañas, Irene; Carrión, Mar; Pérez-García, Selene; Villanueva-Romero, Raúl; Castro, David; Lamana, Amalia; Mellado, Mario ; González-Álvaro, Isidoro; Gomariz, Rosa P.artículo
2closedAccessaccesoRestringido.pdf.jpg1-Sep-2005A framework for computational and experimental methods: Identifying dimerization residues in CCR chemokine receptorsJuan, David de; Mellado, Mario ; Rodríguez Frade, José Miguel; Hernanz-Falcón, Patricia; Serrano, Antonio ; Sol, Antonio del; Valencia, Alfonso; Martínez-A, Carlos CSIC ORCID; Rojas, A. M. CSIC ORCID artículo
3openAccessRODRÍGUEZ-FRADE, J.M. - Scient. Rep. - 2019.pdf.jpg2019Activation of Th lymphocytes alters pattern expression and cellular location of VIP receptors in healthy donors and early arthritis patientsVillanueva-Romero, Raúl; Gutiérrez-Cañas, Irene; Carrión, M.; González-Álvaro, Isidoro; Rodríguez-Frade, José Miguel; Mellado, Mario ; Martínez, C.; Gomariz, Rosa P.; Juarranz, Y.artículo
4openAccess211.pdf.jpgAug-1997The amino-terminal domain of the CCR2 chemokine receptor acts as coreceptor for HIV-1 infectionFrade, José M.R.; Llorente Gómez, María de las Mercedes; Mellado, Mario ; Alcamí, José CSIC ORCID CVN; Gutiérrez Ramos, José Carlos; Zaballos, Ángel; Real, Gustavo del; Martínez-Alonso, Carlosartículo
5closedAccessARTICULO ACCESO_RESTRINGIDO.pdf.jpgJul-2011CD69 modulates sphingosine-1-phosphate-induced migration of skin dendritic cellsLamana, Amalia; Martín, Pilar; Fuente, Hortensia de la; Martínez Muñoz, Laura CSIC ORCID; Cruz-Adalia, Aránzazu; Ramirez-Huesca, Marta; Escribano, Cristina; Gollmer, Kathrin; Mellado, Mario ; Stein, Jens V.; Rodríguez-Fernández, José Luis CSIC ORCID ; Sánchez-Madrid, Francisco; Martínez del Hoyo, Gloriaartículo
6closedAccessaccesoRestringido.pdf.jpg19-May-2020CXCR1 and CXCR2 Chemokine Receptor Agonists Produced by Tumors Induce Neutrophil Extracellular Traps that Interfere with Immune CytotoxicityTeijeira, Alvaro; Garasa, Saray; Gato, María; Alfaro, Carlos; Migueliz, Itziar; Cirella, Assunta; Andrea, Carlos de; Ochoa, Maria Carmen; Otano, Itziar; Etxeberria, Iñaki; Andueza, María Pilar; Nieto, Celia P.; Resano, Leyre; Azpilikueta, Arantza; Allegretti, Marcello; Pizzol, María de; Ponz-Sarvisé, Mariano; Rouzaut, Ana; Sanmamed, Miguel F.; Schalper, Kurt; Carleton, Michael; Mellado, Mario ; Rodriguez-Ruiz, María E.; Berraondo, Pedro; Perez-Gracia, Jose L.; Melero, Ignacioartículo
7closedAccessrestringido.pdf.jpg15-May-2006DC-SIGN ligation on dendritic cells results in ERK and PI3K activation and modulates cytokine productionCaparrós, Esther CSIC; Muñoz, Pilar; Sierra-Filardi, Elena CSIC ORCID ; Serrano-Gómez, Diego; Puig-Kröger, Amaya CSIC ORCID; Rodríguez-Fernández, José Luis CSIC ORCID ; Mellado, Mario ; Sancho, Jaime CSIC ORCID ; Zubiaur, Mercedes CSIC ORCID ; Corbí, Angel L. artículo
8closedAccess15-May-2009Determination of human growth hormone in human serum samples by surface plasmon resonance immunoassayTreviño, Juan CSIC; Calle Martín, Ana CSIC; Rodríguez Frade, José Miguel; Mellado, Mario ; Lechuga, Laura M. CSIC ORCID artículo
9openAccessJ. Biol. Chem.-2001-Robledo-45098-105.pdf.jpg30-Nov-2001Expression of functional chemokine receptors CXCR3 and CXCR4 on human melanoma cellsRobledo, Mar M.; Bartolomé, Rubén Álvaro CSIC ORCID ; Longo, Natividad; Rodríguez-Frade, José Miguel; Mellado, Mario ; Longo, Isabel; van Muijen, Goos N. P.; Sánchez-Mateos, Paloma; Teixidó, Joaquín CSIC ORCID artículo
10openAccess350.pdf.jpgAug-2002Functional Inactivation of CXC Chemokine Receptor 4–mediated Responses through SOCS3 Up-regulationSoriano, Silvia F.; Hernanz-Falcón, Patricia; Rodríguez Frade, José Miguel; Martín de Ana, Ana; Garzón, Ruth; Carvalho-Pinto, Carla E.; Vila-Coro, Antonio J.; Zaballos, Ángel; Balomenos, Dimitrios CSIC ORCID CVN ; Martínez-Alonso, Carlos; Mellado, Mario artículo
11closedAccessaccesoRestringido.pdf.jpg1-Aug-2020Growth Hormone Reprograms Macrophages toward an Anti-Inflammatory and Reparative Profile in an MAFB-Dependent MannerSoler Palacios, Blanca; Nieto, Concha ; González de la Aleja, Arturo ; Andrés, Nuria de; Domínguez-Soto, Ángeles CSIC ; Lucas, Pilar; Cuenda, Ana; Rodríguez-Frade, José Miguel; Martínez-A, Carlos CSIC ORCID; Villares, Ricardo CSIC ORCID ; Corbí, Angel L. ; Mellado, Mario artículo
12openAccessfimmu-11-01336.pdf.jpg2020Identification of a Human SOCS1 Polymorphism That Predicts Rheumatoid Arthritis SeverityLamana, Amalia; Villares, Ricardo CSIC ORCID ; Seoane, Iria V.; Andrés, Nuria de; Lucas, Pilar; Emery, Paul; Vital, Edward M.; Triguero-Martínez, Ana; Márquez, Ana; Ortiz, Ana M.; Maxime, Robin; Martínez, Carmen; Martín, J.; Gomariz, Rosa P.; Ponchel, Frederique; González-Álvaro, Isidoro; Mellado, Mario artículo
13closedAccessaccesoRestringido.pdf.jpg19-Apr-2019Image Processing Protocol for the Analysis of the Diffusion and Cluster Size of Membrane Receptors by Fluorescence MicroscopySorzano, Carlos Óscar S.; Cascio, Graciela; García-Cuesta, Eva María; Vargas, Javier; Mellado, Mario ; Rodríguez, Jose Miguelartículo
14closedAccessaccesoRestringido.pdf.jpg2013Implementation of a SPR immunosensor for the simultaneous detection of the 22K and 20K hGH isoforms in human serum samplesJuan-Franco, Elena de CSIC; Rodríguez Frade, José Miguel; Mellado, Mario ; Lechuga, Laura M. CSIC ORCID artículo
15closedAccessrestringido.pdf.jpg1-Feb-2006Opioids trigger α5β1 integrin-mediated monocyte adhesionPello, Oscar M.; Duthey, Béatrice; García-Bernal, David CSIC ORCID; Rodríguez-Frade, José Miguel; Stein, Jens V.; Teixidó, Joaquín CSIC ORCID ; Martínez-Alonso, Carlos; Mellado, Mario artículo
16openAccess224.pdf.jpgJul-1997Polarization of chemokine receptors to the leading edge during lymphocyte chemotaxisNieto, Marta; Frade, José M.R.; Sancho, David; Mellado, Mario ; Martínez-Alonso, Carlos; Sánchez Madrid, Franciscoartículo
17closedAccessaccesoRestringido.pdf.jpg2013Real-time detection of the chemokine CXCL12 in urine samples by surface plasmon resonanceVega, Beatriz; Calle Martín, Ana CSIC; Lechuga, Laura M. CSIC ORCID ; Armelles Reig, Gaspar CSIC ORCID; Rodríguez Frade, José Miguel; Mellado, Mario artículo
18closedAccessaccesoRestringido.pdf.jpgAug-2018Remodeling our concept of chemokine receptor function: From monomers to oligomersMartínez Muñoz, Laura CSIC ORCID; Villares, Ricardo CSIC ORCID ; Rodríguez-Fernández, José Luis CSIC ORCID ; Rodríguez Frade, José Miguel; Mellado, Mario artículo
19openAccess309.pdf.jpgOct-2000Role of the PI3K regulatory subunit in the control of actin organization and cell migrationJiménez, Concepción; Armas Portela, Rosario; Mellado, Mario ; Rodríguez Frade, José Miguel; Collard, John; Serrano, Antonio ; Martínez-Alonso, Carlos; Ávila, Jesús CSIC ORCID ; Carrera, Ana C.artículo
20closedAccessaccesoRestringido.pdf.jpg5-Apr-2018Separating actin-dependent chemokine receptor nanoclustering from dimerization indicates a role for clustering in CXCR4 signaling and function.Martínez Muñoz, Laura CSIC ORCID; Rodríguez Frade, José Miguel; Barroso, Rubén; Sorzano, Carlos Óscar S.; Torreño-Pina, José A.; Santiago, César CSIC ORCID ; Manzo, Carlo; Lucas, Pilar; García-Cuesta, Eva María; Gutierrez, Enric; Barrios, Laura CSIC; Vargas, Javier; Cascio, Graciela; Carrasco, Yolanda R.; Sánchez Madrid, Francisco; Garcia-Parajo, Maria F.; Mellado, Mario artículo