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Firma en Digital.CSIC (*)
Mellado, Mario
Centro o Instituto
CSIC - Centro Nacional de Biotecnología (CNB)
Inmunología y Oncología
The chemokines are a family of chemoattractant mediators with strong pharmacological interest, they bind to GPCR receptors and direct cell movement. They are essential for immune system homeostasis and key proteins in multiple inflammatory and autoimmune diseases such as rheumatoid arthritis, multiple sclerosis, ulcerative colitis, Crohn’s disease, etc. They have also been linked to HIV-1 infection, tumor metastasis and transplant rejection. After an initial stage studying the signaling cascades triggered by chemokines, he described, for the first time, that these inflammatory mediators activate the JAK/STAT pathway, a signaling pathway classically associated to the cytokine receptors. He also was pioneer describing that chemokine receptors form homo- and heterodimers at the cell surface. At that time, he published more than 50 manuscripts, signed 4 international patents and supervised 4 doctoral theses. In 2005, he obtained a permanent scientific position in the Spanish Research Council (CSIC) and stabilized his position as a group leader in the CNB. He consolidated his research on chemokines and incorporated new innovative imaging technologies, such as those based in resonant energy transfer (FRET and BRET). Using these techniques, he confirmed the existence of chemokine receptor complexes even in the absence of ligands and demonstrated that homo- and heterodimers were dynamic conformations regulated by receptor expression and ligand binding. At this stage, he supervised 8 doctoral theses and became interested in translational aspects of his research. He participated in 2 european consortia supported by the UE FP7 programme that facilitated an international development of his research. He also participated in 2 national consortia on rheumatic diseases, bringing his research closer to clinical and translational medicine. His commitment to incorporate new technologies in his research, led him to start working with TIRF techniques (total internal reflection microscopy) and super-resolution microscopy (STED). Now he uses these methodologies to analyze the spatial distribution and dynamics of the chemokine receptors in the cell membrane, as well as to identify those factors involved in their regulation. He is also interested in the dynamics of the signaling molecules associated to these receptors, as well as in the identification of potential targets and tools with therapeutic value to intervene in chemokine function. Currently he is also director of the CNB/CSIC.
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Results 1-20 of 34 (Search time: 0.047 seconds).

RightsPreviewIssue DateTitleAuthor(s)Type
1openAccessclinical_VIP_axis_inflammatory_autoimmune_diseases.pdf.jpg20-Dec-2019A clinical approach for the use of VIP axis in inflammatory and autoimmune diseasesMartínez, Carmen; Juarranz, Yasmina; Gutiérrez-Cañas, Irene; Carrión, Mar; Pérez-García, Selene; Villanueva-Romero, Raúl; Castro, David; Lamana, Amalia; Mellado, Mario CSIC ORCID ; González-Álvaro, Isidoro; Gomariz, Rosa P.artículo
2closedAccessaccesoRestringido.pdf.jpg1-Sep-2005A framework for computational and experimental methods: Identifying dimerization residues in CCR chemokine receptorsJuan, David CSIC ORCID ; Mellado, Mario CSIC ORCID ; Rodríguez-Frade, José Miguel CSIC ORCID CVN; Hernanz-Falcón, Patricia; Serrano Moral, Antonio CSIC ORCID ; Sol, Antonio del; Valencia, Alfonso; Martínez-A, Carlos CSIC ORCID; Rojas Mendoza, Ana M. CSIC ORCID artículo
3openAccessRODRÍGUEZ-FRADE, J.M. - Scient. Rep. - 2019.pdf.jpg2019Activation of Th lymphocytes alters pattern expression and cellular location of VIP receptors in healthy donors and early arthritis patientsVillanueva-Romero, Raúl; Gutiérrez-Cañas, Irene; Carrión, M.; González-Álvaro, Isidoro; Rodríguez-Frade, José Miguel CSIC ORCID CVN; Mellado, Mario CSIC ORCID ; Martínez, C.; Gomariz, Rosa P.; Juarranz, Y.artículo
4openAccess211.pdf.jpgAug-1997The amino-terminal domain of the CCR2 chemokine receptor acts as coreceptor for HIV-1 infectionFrade, José M.R.; Llorente Gómez, María de las Mercedes; Mellado, Mario CSIC ORCID ; Alcamí, José CSIC ORCID CVN; Gutiérrez Ramos, José Carlos; Zaballos, Ángel; Real, Gustavo del; Martínez-Alonso, Carlosartículo
5closedAccessARTICULO ACCESO_RESTRINGIDO.pdf.jpgJul-2011CD69 modulates sphingosine-1-phosphate-induced migration of skin dendritic cellsLamana, Amalia; Martín, Pilar; Fuente, Hortensia de la; Martínez Muñoz, Laura CSIC ORCID; Cruz-Adalia, Aránzazu; Ramírez-Huesca, Marta; Escribano, Cristina CSIC; Gollmer, Kathrin; Mellado, Mario CSIC ORCID ; Stein, Jens V.; Rodríguez-Fernández, José Luis CSIC ORCID ; Sánchez-Madrid, Francisco; Martínez del Hoyo, Gloriaartículo
6closedAccessaccesoRestringido.pdf.jpg2013CXCL12-mediated murine neural progenitor cell movement Requires PI3Kß activationHolgado, Borja L.; Martínez-Muñoz, Laura; Sánchez Alcañiz, Juan Antonio CSIC ORCID; Lucas, Pilar; Pérez-García, Vicente; Pérez, Gema; Rodríguez-Frade, José Miguel CSIC ORCID CVN; Nieto, Marta; Marín Parra, Óscar CSIC ORCID; Carrasco, Yolanda R.; Carrera, Ana C.; Álvarez-Dolado, Manuel CSIC ORCID CVN; Mellado, Mario CSIC ORCID artículo
7closedAccessaccesoRestringido.pdf.jpg19-May-2020CXCR1 and CXCR2 Chemokine Receptor Agonists Produced by Tumors Induce Neutrophil Extracellular Traps that Interfere with Immune CytotoxicityTeijeira, Alvaro; Garasa, Saray; Gato, María; Alfaro, Carlos; Migueliz, Itziar; Cirella, Assunta; Andrea, Carlos de; Ochoa, Maria Carmen; Otano, Itziar; Etxeberria, Iñaki; Andueza, María Pilar; Nieto, Celia P.; Resano, Leyre; Azpilikueta, Arantza; Allegretti, Marcello; Pizzol, María de; Ponz-Sarvisé, Mariano; Rouzaut, Ana; Sanmamed, Miguel F.; Schalper, Kurt; Carleton, Michael; Mellado, Mario CSIC ORCID ; Rodriguez-Ruiz, María E.; Berraondo, Pedro; Perez-Gracia, Jose L.; Melero, Ignacioartículo
8closedAccessrestringido.pdf.jpg15-May-2006DC-SIGN ligation on dendritic cells results in ERK and PI3K activation and modulates cytokine productionCaparrós, Esther CSIC ORCID; Muñoz, Pilar; Sierra-Filardi, Elena CSIC ORCID ; Serrano-Gómez, Diego; Puig-Kröger, Amaya CSIC ORCID; Rodríguez-Fernández, José Luis CSIC ORCID ; Mellado, Mario CSIC ORCID ; Sancho, Jaime CSIC ORCID ; Zubiaur, Mercedes CSIC ORCID ; Corbí, Angel L. artículo
9closedAccess15-May-2009Determination of human growth hormone in human serum samples by surface plasmon resonance immunoassayTreviño, Juan CSIC; Calle Martín, Ana CSIC; Rodríguez-Frade, José Miguel CSIC ORCID CVN; Mellado, Mario CSIC ORCID ; Lechuga, Laura M. CSIC ORCID artículo
10openAccessJ. Biol. Chem.-2001-Robledo-45098-105.pdf.jpg30-Nov-2001Expression of functional chemokine receptors CXCR3 and CXCR4 on human melanoma cellsRobledo, Mar M. CSIC; Bartolomé, Rubén Álvaro CSIC ORCID ; Longo, Natividad; Rodríguez-Frade, José Miguel CSIC ORCID CVN; Mellado, Mario CSIC ORCID ; Longo, Isabel; van Muijen, Goos N. P.; Sánchez-Mateos, Paloma; Teixidó, Joaquín CSIC ORCID artículo
11openAccess350.pdf.jpgAug-2002Functional Inactivation of CXC Chemokine Receptor 4–mediated Responses through SOCS3 Up-regulationSoriano, Silvia F.; Hernanz-Falcón, Patricia; Rodríguez-Frade, José Miguel CSIC ORCID CVN; Martín de Ana, Ana; Garzón, Ruth; Carvalho-Pinto, Carla E.; Vila-Coro, Antonio J.; Zaballos, Ángel; Balomenos, Dimitrios CSIC ORCID CVN ; Martínez-Alonso, Carlos; Mellado, Mario CSIC ORCID artículo
12openAccess2023 Front Immunol 14-1200259.pdf.jpg5-Jul-2023Growth hormone remodels the 3D-structure of the mitochondria of inflammatory macrophages and promotes metabolic reprogrammingSoler Palacios, Blanca; Villares, Ricardo CSIC ORCID ; Lucas, Pilar; Rodríguez-Frade, José Miguel CSIC ORCID CVN; Cayuela, Ana; Piccirillo, Jonathan G.; Lombardia, Manuel; Delgado Gestoso, David; Fernández-García, Miguel; Risco Ortiz, Cristina ; Barbas, Coral; Corrales, Fernando J. CSIC ORCID ; Sorzano, Carlos Óscar S.; Martínez-Martín, Nuria CSIC ORCID; Conesa, José Javier; Iborra, Francisco J. CSIC ORCID ; Mellado, Mario CSIC ORCID artículo
13closedAccessaccesoRestringido.pdf.jpg1-Aug-2020Growth Hormone Reprograms Macrophages toward an Anti-Inflammatory and Reparative Profile in an MAFB-Dependent MannerSoler Palacios, Blanca; Nieto, Concha ; González de la Aleja, Arturo CSIC ORCID ; Andrés, Nuria de; Domínguez-Soto, Ángeles CSIC ; Lucas, Pilar; Cuenda, Ana; Rodríguez-Frade, José Miguel CSIC ORCID CVN; Martínez-A, Carlos CSIC ORCID; Villares, Ricardo CSIC ORCID ; Corbí, Angel L. ; Mellado, Mario CSIC ORCID artículo
14openAccessfimmu-11-01336.pdf.jpg2020Identification of a Human SOCS1 Polymorphism That Predicts Rheumatoid Arthritis SeverityLamana, Amalia; Villares, Ricardo CSIC ORCID ; Seoane, Iria V.; Andrés, Nuria de; Lucas, Pilar; Emery, Paul; Vital, Edward M.; Triguero-Martínez, Ana; Márquez, Ana; Ortiz, Ana M.; Maxime, Robin; Martínez, Carmen; Martín, J.; Gomariz, Rosa P.; Ponchel, Frederique; González-Álvaro, Isidoro; Mellado, Mario CSIC ORCID artículo
15closedAccessaccesoRestringido.pdf.jpg19-Apr-2019Image Processing Protocol for the Analysis of the Diffusion and Cluster Size of Membrane Receptors by Fluorescence MicroscopySorzano, Carlos Óscar S.; Cascio, Graciela; García-Cuesta, Eva María; Vargas, Javier; Mellado, Mario CSIC ORCID ; Rodríguez, Jose Miguelartículo
16closedAccessaccesoRestringido.pdf.jpg2013Implementation of a SPR immunosensor for the simultaneous detection of the 22K and 20K hGH isoforms in human serum samplesJuan-Franco, Elena de CSIC; Rodríguez-Frade, José Miguel CSIC ORCID CVN; Mellado, Mario CSIC ORCID ; Lechuga, Laura M. CSIC ORCID artículo
17closedAccessrestringido.pdf.jpg1-Feb-2006Opioids trigger α5β1 integrin-mediated monocyte adhesionPello, Oscar M. CSIC ORCID; Duthey, Béatrice; García-Bernal, David CSIC ORCID; Rodríguez-Frade, José Miguel CSIC ORCID CVN; Stein, Jens V.; Teixidó, Joaquín CSIC ORCID ; Martínez-Alonso, Carlos; Mellado, Mario CSIC ORCID artículo
18openAccess224.pdf.jpgJul-1997Polarization of chemokine receptors to the leading edge during lymphocyte chemotaxisNieto, Marta; Frade, José M.R.; Sancho, David; Mellado, Mario CSIC ORCID ; Martínez-Alonso, Carlos; Sánchez Madrid, Franciscoartículo
19openAccess520279.pdf.jpg2022Prolactin Reduces Hippocampal Parvalbumin and GABAAReceptor Expression in Female MiceMellado, S. ; Moreno-Ruiz, B.; Expósito, Sara CSIC ORCID; Fernández-García, Marcos CSIC ORCID ; Martín, E.D.artículo
20closedAccessaccesoRestringido.pdf.jpg2013Real-time detection of the chemokine CXCL12 in urine samples by surface plasmon resonanceVega, Beatriz; Calle Martín, Ana CSIC; Lechuga, Laura M. CSIC ORCID ; Armelles Reig, Gaspar CSIC ORCID; Rodríguez-Frade, José Miguel CSIC ORCID CVN; Mellado, Mario CSIC ORCID artículo