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closedAccessaccesoRestringido.pdf.jpg2004A simple strategy for the purification of large thermophilic proteins overexpressed in mesophilic microorganisms: Application to multimeric enzymes from Thermus sp. strain T2 expressed in Escherichia coliPessela, Benevides C.  ; Torres, Rodrigo; Fuentes, Manuel  ; Mateo, César; Filho, Miguel; Carrascosa, Alfonso V. ; Vian Herrero, Alejandro; García, José Luis ; Guisán, José M.; Fernández-Lafuente, Roberto Artículo
closedAccess13-Feb-2009Activation of bacterial thermoalkalophilic lipases is spurred by dramatic structural rearrangementsCarrasco-López, César ; Godoy, César A. ; Rivas, Blanca de las ; Fernández-Lorente, Gloria  ; Palomo, José Miguel ; Guisán, José Manuel ; Fernández-Lafuente, Roberto ; Martínez-Ripoll, Martín ; Hermoso, Juan A. Artículo
openAccessmolecules-21-01577.pdf.jpg19-Nov-2016Agarose and Its Derivatives as Supports for Enzyme ImmobilizationZucca, Paolo; Fernández-Lafuente, Roberto ; Sanjust, EnricoArtículo
openAccessWO_2004_051278_A1.pdf.jpg17-Jun-2004Antibodies and antigens immobilised on magnetic silica particles used as biosensorsFuentes García, Manuel; Mateo González, César ; Fernández-Lafuente, Roberto ; Guisán, José Manuel ; Forces Redondo, Carmen; Rodríguez Galván, Ignacio; Tartaj, Pedro ; Serna Pereda, Carlos J. Patente
openAccess2208121_B1.pdf.jpg16-Aug-2005Anticuerpos y antígenos inmovilizados sobre partículas magnéticas de sílice como biosensoresFuentes García, Manuel; Mateo González, César ; Fernández-Lafuente, Roberto ; Guisán, José Manuel ; Forces Redondo, Carmen; Rodríguez Galván, Ignacio; Tartaj, Pedro ; Serna Pereda, Carlos J. Patente
openAccessPostprint_2015_JMolCatB_V117_P38.pdf.jpg2015Bovine trypsin immobilization on agarose activated with divinylsulfone: Improved activity and stability via multipoint covalent attachmentDos Santos, José C. S.; Rueda, Nazzoly; Barbosa, Oveimar; Millán-Linares, María del Carmen  ; Pedroche, Justo  ; Yuste, María del Mar; Gonçalves, Luciana R. B.; Fernández-Lafuente, Roberto Artículo
closedAccessaccesoRestringido.pdf.jpg2015Characterization of supports activated with divinyl sulfone as a tool to immobilize and stabilize enzymes via multipoint covalent attachment. Application to chymotrypsinDos Santos, José C. S.; Barbosa, Oveimar; Fernández-Sánchez, Jorge F.; Medina-Castillo, Antonio L.; Ramón-Márquez, Teresa; Arias-Martos, María C.; Millán-Linares, María del Carmen  ; Pedroche, Justo  ; Yust, María del Mar ; Gonçalves, Luciana R. B.; Fernández-Lafuente, Roberto ; Rueda, NazzolyArtículo
closedAccess13-Apr-2009Coating of Soluble and Immobilized Enzymes with Ionic Polymers: Full Stabilization of the Quaternary Structure of Multimeric EnzymesBolívar Bolívar, Juan Manuel ; Rocha-Martín, Javier  ; Mateo, César; Cava, Felipe ; Berenguer, José ; Fernández-Lafuente, Roberto ; Guisán, José Manuel Artículo
openAccessUltrasound_Alves.pdf.jpg7-Jul-2014Combined effects of ultrasound and immobilization protocol on butyl acetate synthesis catalyzed by CALBAlves, Joana S.; García-Galán, Cristina; Schein, Mirela F.; Silva, Alexandre M.; Barbosa, Oveimar; Ayub, Marco A. Z.; Fernández-Lafuente, Roberto ; Rodrigues, Rafael C.Artículo
openAccess12866_2015_Article_423.pdf.jpg29-Apr-2015Comparing methods of determining Legionella spp. in complex water matricesDíaz-Flores, Álvaro; Montero, Juan C.; Castro, Francisco J.; Alejandres, Eva M.; Bayón, Carmen; Solís, Inmaculada; Fernández-Lafuente, Roberto ; Rodríguez, GuillermoArtículo
closedAccess2008Crystallization and preliminary X-ray diffraction studies of the BTL2 lipase from extremophilic microorganism Bacillus thermocatenulatusCarrasco-López, César ; Godoy, César; Rivas, Blanca de las ; Fernández-Lorente, Gloria  ; Palomo, José Miguel ; Guisán Seijas, José Manuel; Fernández-Lafuente, Roberto ; Martínez-Ripoll, Martín ; Hermoso, Juan A. Artículo
openAccess2018Design of bactericidal peptides against Escherichia coli O157:H7, Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureusCruz, Jenniffer; Rondón-Villarreal, Paola; Rodrigo G. Torres; Urquiza, Mauricio; Guzmán, Fanny; Álvarez, Claudio; Abengozar, M. A. ; Sierra, Daniel A.; Rivas, Luis ; Fernández-Lafuente, Roberto ; Ortiz, ClaudiaArtículo
openAccessmolecules-22-00091.pdf.jpg5-Jan-2017Desorption of Lipases Immobilized on Octyl-Agarose Beads and Coated with Ionic Polymers after Thermal Inactivation. Stronger Adsorption of Polymers/Unfolded Protein CompositesVirgen-Ortíz, José J.; Pedrero, Sara G.; Fernández-López, Laura; López-Carrobles, Nerea; Gorines, Beatriz C.; Otero Hernández, Cristina ; Fernández-Lafuente, Roberto Artículo
openAccessEnzyme_Immobilization_Fernandez-Lafuente.pdf.jpg10-Dec-2014Editorial: Special issue - Enzyme immobilizationFernández-Lafuente, Roberto Artículo
openAccessmolecules-23-02993.pdf.jpg16-Nov-2018Evaluation of strategies to produce highly porous cross-linked aggregates of porcine pancreas lipase with magnetic propertiesGuimarães, José Renato; Giordano, Raquel de Lima Camargo; Fernández-Lafuente, Roberto ; Tardioli, Paulo W.Artículo
openAccessStyrene-Divinylbenzene_Beads_Garcia-Galan.pdf.jpg10-Jun-2014Evaluation of styrene-divinylbenzene beads as a support to immobilize lipasesGarcía-Galán, Cristina; Barbosa, Oveimar; Hernández Sánchez, Karel; Dos Santos, José C. S.; Rodrigues, Rafael C.; Fernández-Lafuente, Roberto Artículo
closedAccessaccesoRestringido.pdf.jpg3-May-2015Evaluation of supports activated with divinylsulfone to the stabilization of chymotrypsinRueda, Nazzoly; Sousa dos Santos, José Cleiton; Barbosa, Oveimar; Gonçalves, Luciana R.B.; Pedroche, Justo  ; Yust, María del Mar ; Millán-Linares, María del Carmen  ; Fernández-Lafuente, Roberto Comunicación de congreso
openAccesscatalysts-07-00250-v2.pdf.jpg25-Aug-2017Exploiting the Versatility of Aminated Supports Activated with Glutaraldehyde to Immobilize β-galactosidase from Aspergillus oryzaeZaak, Hadjer; Peirce, Sara; Albuquerque, Tiago L.; Sassi, Mohamed; Fernández-Lafuente, Roberto Artículo
openAccess1471-2180-13-88.pdf.jpg22-Apr-2013Fast immunosensing technique to detect Legionella pneumophila in different natural and anthropogenic environments: comparative and collaborative trialsBedrina, Begoña; Macián, Sonia; Solís, Inmaculada; Fernández-Lafuente, Roberto ; Baldrich, Eva; Rodríguez, GuillermoArtículo
openAccessmolecules-23-03188-v2.pdf.jpg3-Dec-2018Further stabilization of alcalase immobilized on glyoxyl supports: amination plus modification with glutaraldehydeHussain, Fouzia; Arana-Peña, Sara; Morellon-Sterling, Roberto; Barbosa, Oveimar; Ait Braham, Sabrina; Kamal, Shagufta; Fernández-Lafuente, Roberto Artículo